Chiral Flavanones from Amygdalus lycioides Spach: Structural Elucidation and Identification of TNFalpha Inhibitors by Bioactivity-guided Fractionation
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental
3.1. Chemicals and Instrumentations
3.2. Plant Material
3.3. Extraction and Fractionation
3.4. Chromatography
3.4.1. Analytical HPLC-UV/PAD/CD
3.4.2. Semipreparative HPLC
3.5. Biological Assays
3.5.1. In Vitro anti-TNFα Assay
3.5.2. In Vivo anti-TNFα Assay
3.5.3. Cytotoxicity Assay
4. Conclusions
Supplementary Materials
Acknowledgments
- Sample Availability: Samples of the compounds 1–4 are available from the authors.
References and Notes
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Gaggeri, R.; Rossi, D.; Christodoulou, M.S.; Passarella, D.; Leoni, F.; Azzolina, O.; Collina, S. Chiral Flavanones from Amygdalus lycioides Spach: Structural Elucidation and Identification of TNFalpha Inhibitors by Bioactivity-guided Fractionation. Molecules 2012, 17, 1665-1674. https://doi.org/10.3390/molecules17021665
Gaggeri R, Rossi D, Christodoulou MS, Passarella D, Leoni F, Azzolina O, Collina S. Chiral Flavanones from Amygdalus lycioides Spach: Structural Elucidation and Identification of TNFalpha Inhibitors by Bioactivity-guided Fractionation. Molecules. 2012; 17(2):1665-1674. https://doi.org/10.3390/molecules17021665
Chicago/Turabian StyleGaggeri, Raffaella, Daniela Rossi, Michael S. Christodoulou, Daniele Passarella, Flavio Leoni, Ornella Azzolina, and Simona Collina. 2012. "Chiral Flavanones from Amygdalus lycioides Spach: Structural Elucidation and Identification of TNFalpha Inhibitors by Bioactivity-guided Fractionation" Molecules 17, no. 2: 1665-1674. https://doi.org/10.3390/molecules17021665