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Molecules 2012, 17(12), 14968-14974; doi:10.3390/molecules171214968
Communication

Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography

1,2, 2, 1, 3, 2 and 2,*
Received: 31 October 2012; in revised form: 27 November 2012 / Accepted: 11 December 2012 / Published: 17 December 2012
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Abstract: pH-Zone-refining counter-current chromatography (pH-zone-refining CCC) was successfully applied for the large-scale separation of alkaloids from Corydalis bungeana. The crude extract was separated by a two-phase solvent system composed of petroleum ether–ethyl acetate–methanol–water (5:5:2:8, v/v) where triethylamine (10 mM) was added to the upper organic stationary phase as a retainer and hydrochloric acid (5 mM) to the aqueous mobile phase as a displacer. As a result, 285 mg of protopine, 86 mg of corynoloxine, 430 mg of coryno1ine, and 115 mg of acetylcorynoline were obtained from 3.0 g of crude extract in a one-step separation. The purities of these compounds were 99.1%, 98.3%, 99.0% and 98.5%, respectively, as determined by HPLC. The chemical structures of these isolated compounds were confirmed by ESI-MS, 1H-NMR and 13C-NMR.
Keywords: pH-zone-refining counter-current chromatography; large-scale separation; Corydalis bungeana; alkaloids pH-zone-refining counter-current chromatography; large-scale separation; Corydalis bungeana; alkaloids
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, X.; Dong, H.; Shu, X.; Zheng, Z.; Yang, B.; Huang, L. Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography. Molecules 2012, 17, 14968-14974.

AMA Style

Wang X, Dong H, Shu X, Zheng Z, Yang B, Huang L. Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography. Molecules. 2012; 17(12):14968-14974.

Chicago/Turabian Style

Wang, Xiao; Dong, Hongjing; Shu, Xikai; Zheng, Zhenjia; Yang, Bin; Huang, Luqi. 2012. "Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography." Molecules 17, no. 12: 14968-14974.


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