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Molecules 2012, 17(11), 13569-13591; doi:10.3390/molecules171113569

Nucleoside Triphosphates — Building Blocks for the Modification of Nucleic Acids

Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland
Received: 21 September 2012 / Revised: 7 November 2012 / Accepted: 9 November 2012 / Published: 15 November 2012
(This article belongs to the Special Issue DNA-Directed Chemistry)
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Abstract

Nucleoside triphosphates are moldable entities that can easily be functionalized at various locations. The enzymatic polymerization of these modified triphosphate analogues represents a versatile platform for the facile and mild generation of (highly) functionalized nucleic acids. Numerous modified triphosphates have been utilized in a broad palette of applications spanning from DNA-tagging and -labeling to the generation of catalytic nucleic acids. This review will focus on the recent progress made in the synthesis of modified nucleoside triphosphates as well as on the understanding of the mechanisms underlying their polymerase acceptance. In addition, the usefulness of chemically altered dNTPs in SELEX and related methods of in vitro selection will be highlighted, with a particular emphasis on the generation of modified DNA enzymes (DNAzymes) and DNA-based aptamers.
Keywords: modified nucleoside triphosphates; DNA polymerases; SELEX; DNAzymes; PEX; chemically modified nucleic acids modified nucleoside triphosphates; DNA polymerases; SELEX; DNAzymes; PEX; chemically modified nucleic acids
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Hollenstein, M. Nucleoside Triphosphates — Building Blocks for the Modification of Nucleic Acids. Molecules 2012, 17, 13569-13591.

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