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Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier
Center for Systems and Synthetic Biology, Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 21 September 2012; in revised form: 31 October 2012 / Accepted: 1 November 2012 / Published: 6 November 2012
Abstract: DNA circuits have proven to be useful amplifiers for diagnostic applications, in part because of their modularity and programmability. In order to determine whether different circuits could be modularly stacked, we used a catalytic hairpin assembly (CHA) circuit to initiate a hybridization chain reaction (HCR) circuit. In response to an input nucleic acid sequence, the CHA reaction accumulates immobilized duplexes and HCR elongates these duplexes. With fluorescein as a reporter each of these processes yielded 10-fold signal amplification in a convenient 96-well format. The modular circuit connections also allowed the output reporter to be readily modified to a G-quadruplex-DNAzyme that yielded a fluorescent signal.
Keywords: catalyzed hairpin assembly (CHA); hybridization chain reaction (HCR); 96-well plate; enzyme-free
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Jiang, Y.; Li, B.; Chen, X.; Ellington, A.D. Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier. Molecules 2012, 17, 13211-13220.
Jiang Y, Li B, Chen X, Ellington AD. Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier. Molecules. 2012; 17(11):13211-13220.
Jiang, Yu; Li, Bingling; Chen, Xi; Ellington, Andrew D. 2012. "Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier." Molecules 17, no. 11: 13211-13220.