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Molecules 2012, 17(11), 13211-13220; doi:10.3390/molecules171113211

Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier

Center for Systems and Synthetic Biology, Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 21 September 2012 / Revised: 31 October 2012 / Accepted: 1 November 2012 / Published: 6 November 2012
(This article belongs to the Special Issue Diversity Oriented Synthesis)
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DNA circuits have proven to be useful amplifiers for diagnostic applications, in part because of their modularity and programmability. In order to determine whether different circuits could be modularly stacked, we used a catalytic hairpin assembly (CHA) circuit to initiate a hybridization chain reaction (HCR) circuit. In response to an input nucleic acid sequence, the CHA reaction accumulates immobilized duplexes and HCR elongates these duplexes. With fluorescein as a reporter each of these processes yielded 10-fold signal amplification in a convenient 96-well format. The modular circuit connections also allowed the output reporter to be readily modified to a G-quadruplex-DNAzyme that yielded a fluorescent signal.
Keywords: catalyzed hairpin assembly (CHA); hybridization chain reaction (HCR); 96-well plate; enzyme-free catalyzed hairpin assembly (CHA); hybridization chain reaction (HCR); 96-well plate; enzyme-free
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Jiang, Y.; Li, B.; Chen, X.; Ellington, A.D. Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier. Molecules 2012, 17, 13211-13220.

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