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• Jiang, Y
• Li, B
• Chen, X
• Ellington, AD.
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• Li, B
• Chen, X
• Ellington, AD.
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• Li, B
• Chen, X
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Molecules 2012, 17(11), 13211-13220; doi:10.3390/molecules171113211

Article

Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier

Yu Jiang ^† , Bingling Li ^† , Xi Chen and Andrew D. Ellington*

Center for Systems and Synthetic Biology, Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA ^† These authors contributed equally to this work.

* Author to whom correspondence should be addressed.

Received: 21 September 2012; in revised form: 31 October 2012 / Accepted: 1 November 2012 / Published: 6 November 2012

(This article belongs to the Special Issue Diversity Oriented Synthesis)

Download PDF Full-Text [763 KB, uploaded 6 November 2012 10:32 CET]

Abstract: DNA circuits have proven to be useful amplifiers for diagnostic applications, in part because of their modularity and programmability. In order to determine whether different circuits could be modularly stacked, we used a catalytic hairpin assembly (CHA) circuit to initiate a hybridization chain reaction (HCR) circuit. In response to an input nucleic acid sequence, the CHA reaction accumulates immobilized duplexes and HCR elongates these duplexes. With fluorescein as a reporter each of these processes yielded 10-fold signal amplification in a convenient 96-well format. The modular circuit connections also allowed the output reporter to be readily modified to a G-quadruplex-DNAzyme that yielded a fluorescent signal.

Keywords: catalyzed hairpin assembly (CHA); hybridization chain reaction (HCR); 96-well plate; enzyme-free

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