Abstract: A separation method was developed for the preparative separation and enrichment of the non-caloric sweetener mogroside V from Siraitia grosvenorii. The adsorption properties of six macroporous resins were evaluated. Results showed that HZ 806 resin offered the best adsorption and desorption capacities. Based on the adsorption experiments on HZ 806, the adsorption data were found to fit the Freundlich model well. The pseudo-second-order kinetic model showed the highest correlation with the experimental results. Separation was performed with deionized water and 40% aqueous ethanol solution as mobile phases. In a typical run, 100 g of herb was processed and 3.38 g of mogroside V with a purity of 10.7% was harvested. This separation method provided a 15.1-fold increase in the purification factor from 0.5% to 10.7%. The present study showed that HZ 806 resins were effective for the separation and enrichment of mogroside V from S. grosvenorii.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Zhang, M.; Yang, H.; Zhang, H.; Wang, Y.; Hu, P. Development of a Process for Separation of Mogroside V from Siraitia grosvenorii by Macroporous Resins. Molecules 2011, 16, 7288-7301.
Zhang M, Yang H, Zhang H, Wang Y, Hu P. Development of a Process for Separation of Mogroside V from Siraitia grosvenorii by Macroporous Resins. Molecules. 2011; 16(9):7288-7301.
Zhang, Min; Yang, Huihua; Zhang, Hongyang; Wang, Yuerong; Hu, Ping. 2011. "Development of a Process for Separation of Mogroside V from Siraitia grosvenorii by Macroporous Resins." Molecules 16, no. 9: 7288-7301.