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Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II)
Department of Analytical Chemistry, Faculty of Chemistry and Technology, Teslina 10/V, 21000 Split, Croatia
Department of Mediterranean Agriculture and Aquaculture, University of Zadar, Mihovila Pavlinovića bb, 23000 Zadar, Croatia
* Author to whom correspondence should be addressed.
Received: 18 July 2011; in revised form: 16 August 2011 / Accepted: 17 August 2011 / Published: 25 August 2011
Abstract: We describe a new method using flow-injection analysis with spectro-photometric detection, suitable for the determination of N-acetyl-L-cysteine (NAC). The proposed method is appropriate for the determination of NAC in reaction with Pd2+ ions in the concentration range from 1.0 × 10−5 mol L−1 to 6.0 × 10−5 mol L−1. The detection limit NAC was 5.84 × 10−6 mol L−1 and the recorded relative standard deviation of the method is in the range from 1.67 to 4.11%. NAC and Pd2+ form complexes of Pd2+:NAC molar ratios of 1:1 and 1:2, depending on the ratio of their analytical concentrations. The cumulative conditional stability constant for the Pd(NAC)22+ complex is β12' = 2.69 × 109 L2 mol−2. The proposed method was compared with the classic spectrophotometric determination of NAC, using the same reagent, PdCl2, and had shown certain advantages: a) shorter analysis time; b) the use of smaller volumes of sample and reagents, which make the proposed method cheaper and faster for NAC determination in real samples without sample pretreatment.
Keywords: flow-injection; spectrophotometry; N-Acetyl-L-cysteine; palladium; complexometry
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Giljanović, J.; Brkljača, M.; Prkić, A. Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II). Molecules 2011, 16, 7224-7236.
Giljanović J, Brkljača M, Prkić A. Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II). Molecules. 2011; 16(9):7224-7236.
Giljanović, Josipa; Brkljača, Mia; Prkić, Ante. 2011. "Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II)." Molecules 16, no. 9: 7224-7236.