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Molecules 2011, 16(8), 7171-7182; doi:10.3390/molecules16087171
Article

Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid

1, 2, 1, 3, 1, 1 and 1,*
Received: 29 June 2011; in revised form: 16 August 2011 / Accepted: 17 August 2011 / Published: 23 August 2011
(This article belongs to the Special Issue Enzyme-Catalyzed Reactions)
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Abstract: Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing [bmim]BF4. The three variables studied (molar ratio of hesperidin to decanoic acid, [bmim]BF4/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using [bmim]BF4 and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%.
Keywords: Candida antarctica; lipase; hesperidin; acylation Candida antarctica; lipase; hesperidin; acylation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Araújo, M.E.M.B.; Contesini, F.J.; Franco, Y.E.M.; Sawaya, A.C.F.; Alberto, T.G.; Dalfré, N.; Carvalho, P.O. Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid. Molecules 2011, 16, 7171-7182.

AMA Style

Araújo MEMB, Contesini FJ, Franco YEM, Sawaya ACF, Alberto TG, Dalfré N, Carvalho PO. Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid. Molecules. 2011; 16(8):7171-7182.

Chicago/Turabian Style

Araújo, Maria Elisa Melo Branco de; Contesini, Fabiano Jares; Franco, Yollanda Edwirges Moreira; Sawaya, Alexandra C.H. Frankland; Alberto, Thiago Grando; Dalfré, Natália; Carvalho, Patrícia de Oliveira. 2011. "Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid." Molecules 16, no. 8: 7171-7182.



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