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Molecules 2011, 16(8), 7171-7182; doi:10.3390/molecules16087171

Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid

1, 2, 1, 3, 1, 1 and 1,*
1 São Francisco University, São Francisco de Assis Av., 218, Bragança Paulista, SP 12916-900, Brazil 2 Laboratory of Food Biochemistry, Department of Food Science, Faculty of Food Engineering, State University of Campinas (UNICAMP), Monteiro Lobato Street, 80, Campinas 13083-862, Brazil 3 Department of Plant Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas 13083-970, Brazil
* Author to whom correspondence should be addressed.
Received: 29 June 2011 / Revised: 16 August 2011 / Accepted: 17 August 2011 / Published: 23 August 2011
(This article belongs to the Special Issue Enzyme-Catalyzed Reactions)
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Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing [bmim]BF4. The three variables studied (molar ratio of hesperidin to decanoic acid, [bmim]BF4/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using [bmim]BF4 and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%.
Keywords: Candida antarctica; lipase; hesperidin; acylation Candida antarctica; lipase; hesperidin; acylation
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Araújo, M.E.M.B.; Contesini, F.J.; Franco, Y.E.M.; Sawaya, A.C.F.; Alberto, T.G.; Dalfré, N.; Carvalho, P.O. Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid. Molecules 2011, 16, 7171-7182.

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