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Activity and Stability Studies of Verbascoside, a Novel Antioxidant, in Dermo-Cosmetic and Pharmaceutical Topical Formulations
Department of Pharmaceutical Sciences, University of Ferrara, Via Fossato di Mortara 17-19, 44121 Ferrara, Italy
Ambrosialab, University of Ferrara, Via Mortara 171, 44121 Ferrara, Italy
Biotechnological Research Institute (I.R.B.), Via Lago di Tovel, 7, 36077 Altavilla Vicentina (VI), Italy
* Author to whom correspondence should be addressed.
Received: 27 June 2011; in revised form: 10 August 2011 / Accepted: 11 August 2011 / Published: 18 August 2011
Abstract: We here report the results of our investigations carried out on verbascoside, a phenylpropanoid glycoside known for its antioxidant, anti-inflammatory and photoprotective actions. Verbascoside was obtained from Buddleia davidii meristematic cells, obtained in turn using a sustainable biotechnology platform which employs an in vitro plant cell culture technology. Verbascoside was first investigated to assess the behaviour of the active ingredient in solution or in finished preparations, in view of its potential topical use, especially in skin protection. Stability studies were performed by HPLC, and a PCL assay was adopted to determine the radical scavenging activity toward superoxide anion. The high hydrophilic character of verbascoside, suggested in a somewhat limited range of possible applications, leading us to explore its derivatization to obtain the semi-synthetic derivative VPP, an acyl derivative of verbascoside, with an improved range of applications due to its lower hydrophilic profile. Alone, VPP revealed increased antioxidant activity, both as an active ingredient and in dermocosmetic preparations. Stability studies showed a greater stability of VPP in lipophilic vehicles, whereas the parent verbascoside proved more stable in an O/W emulsions. Verbascoside was also stable in suppositories, an interesting pharmaceutical form for possible applications in treatment of inflammation of the intestinal mucosa.
Keywords: verbascoside; VPP; antioxidant activity; PCL; HPLC analysis; stability in dermocosmetic formulations; suppositories; sustainability; meristematic cells
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Vertuani, S.; Beghelli, E.; Scalambra, E.; Malisardi, G.; Copetti, S.; Toso, R.D.; Baldisserotto, A.; Manfredini, S. Activity and Stability Studies of Verbascoside, a Novel Antioxidant, in Dermo-Cosmetic and Pharmaceutical Topical Formulations. Molecules 2011, 16, 7068-7080.
Vertuani S, Beghelli E, Scalambra E, Malisardi G, Copetti S, Toso RD, Baldisserotto A, Manfredini S. Activity and Stability Studies of Verbascoside, a Novel Antioxidant, in Dermo-Cosmetic and Pharmaceutical Topical Formulations. Molecules. 2011; 16(8):7068-7080.
Vertuani, Silvia; Beghelli, Erika; Scalambra, Emanuela; Malisardi, Gemma; Copetti, Stefano; Toso, Roberto Dal; Baldisserotto, Anna; Manfredini, Stefano. 2011. "Activity and Stability Studies of Verbascoside, a Novel Antioxidant, in Dermo-Cosmetic and Pharmaceutical Topical Formulations." Molecules 16, no. 8: 7068-7080.