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Molecules 2011, 16(8), 7043-7057; doi:10.3390/molecules16087043
Article

Development of a Highly Sensitive and Specific Immunoassay for Determining Chrysoidine, A Banned Dye, in Soybean Milk Film

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Received: 18 July 2011 / Revised: 7 August 2011 / Accepted: 11 August 2011 / Published: 17 August 2011
(This article belongs to the Section Organic Synthesis)
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Abstract

A highly specific and sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA)was developed for the first time for the detection of chrysoidine, a dye banned in soybean milk film. Two haptens with different spacer arms were synthesized to produce antibodies. Both homologous and heterologous immunoassay formats were compared to enhance the icELISA sensitivity. The heterologous icELISA exhibited better performance, with an IC50 (50% inhibitory concentration) of 0.33 ng/mL, a limit of detection (LOD, 10% inhibitory concentration) of 0.04 ng/mL, and a limit of quantitation (LOQ, 20%–80% inhibitory concentration) from 0.09 to 4.9 ng/mL. The developed icELISA was high sensitive and specific, and was applied to determine chrysoidine in fortified soybean milk film samples. The results were in good agreement with that obtained by high-performance liquid chromatography (HPLC) analyses.
Keywords: chrysoidine; antibody; ELISA; soybean milk film chrysoidine; antibody; ELISA; soybean milk film
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lei, H.; Liu, J.; Song, L.; Shen, Y.; Haughey, S.A.; Guo, H.; Yang, J.; Xu, Z.; Jiang, Y.; Sun, Y. Development of a Highly Sensitive and Specific Immunoassay for Determining Chrysoidine, A Banned Dye, in Soybean Milk Film. Molecules 2011, 16, 7043-7057.

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