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Metabolism Study of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 of Radix Panax Notoginseng in Zebrafish
Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Street, Nanjing 210028, China
Key Laboratory of Modern Chinese Medicines and Department of Pharmacognosy, China Pharmaceutical University, 24 Tongjia Lane, Nanjing 210009, China
Nanjing Medical University, Affiliated Nanjing First Hospital, Lab of Clinical Pharmacology, 68 Changle Road, Nanjing 210006, China
* Author to whom correspondence should be addressed.
Received: 4 July 2011; in revised form: 22 July 2011 / Accepted: 29 July 2011 / Published: 5 August 2011
Abstract: Zebrafish, a common model organism for studies of vertebrate development and gene function, has been used in pharmaceutical research as a new and powerful tool in recent years. In the present study, we applied zebrafish for the first time in a metabolic study of notoginsenoside (R1), ginsenoside (Rg1) and ginsenoside (Rb1), which are saponins isolated from Panax notoginseng. Metabolites of these three saponin compounds in zebrafish after exposure for 24 h were identified by high performance liquid chromatography - electrospray mass spectrometry (HPLC-ESI-MS) with a Zorbax C-18 column for separation using a binary gradient elution of 0.05% formic acid acetonitrile - 0.05% formic acid water. The quasi-molecular ions of compounds were detected in negative mode. Step-wise deglycosylation metabolites and hydroxylation metabolites of the three saponins were found, which were coincide with regular methods for metabolic analysis. Our study demonstrated that the zebrafish model can successfully imitate the current metabolic model with advantages of lower cost, far less amount of compound needed, easy set up and high performance. Our data suggests that the zebrafish metabolic model has the potential for developing a novel method for quickly predicting the metabolism of Chinese herb components, including those of trace compounds.
Keywords: zebrafish; notoginsenoside R1; ginsenoside Rg1; ginsenoside Rb1; metabolism
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Wei, Y.; Li, P.; Fan, H.; Peng, Y.; Liu, W.; Wang, C.; Shu, L.; Jia, X. Metabolism Study of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 of Radix Panax Notoginseng in Zebrafish. Molecules 2011, 16, 6621-6633.
Wei Y, Li P, Fan H, Peng Y, Liu W, Wang C, Shu L, Jia X. Metabolism Study of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 of Radix Panax Notoginseng in Zebrafish. Molecules. 2011; 16(8):6621-6633.
Wei, Yingjie; Li, Ping; Fan, Hongwei; Peng, Yunru; Liu, Wei; Wang, Changmei; Shu, Luan; Jia, Xiaobin. 2011. "Metabolism Study of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 of Radix Panax Notoginseng in Zebrafish." Molecules 16, no. 8: 6621-6633.