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Anticancer Activity of Chamaejasmine: Effect on Tubulin Protein
AbstractIn this work, the anticancer activity of chamaejasmine was studied by evaluating its in vitro cytotoxicity against several human cancer cell lines (MCF-7, A549, SGC-7901, HCT-8, HO-4980, Hela, HepG2, PC-3, LNCap, Vero and MDCK) using the MTT assay. Results indicated chamaejasmine showed more notable anticancer activity than taxol against PC-3 cells, with IC50 values of 2.28 and 3.98 µM, respectively. Furthermore, Western blot analysis showed that chamaejasmine was able to increase the expression of β-tubulin, but not α-tubulin. In silico simulations indicated that chamaejasmine specifically interacts with the active site which is located at the top of β-tubulin, thanks to the presence of strong hydrophobic effects between the core templates and the hydrophobic surface of the TB active site. The binding energy (Einter) was calculated to be −164.77 kcal·mol−1. Results presented here suggest that chamaejasmine possesses anti-cancer properties relating to β-tubulin depolymerization inhibition, and therefore is a potential source of anticancer leads for the pharmaceutical industry.
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Fang, W.; Liu, S.; Nie, Y. Anticancer Activity of Chamaejasmine: Effect on Tubulin Protein. Molecules 2011, 16, 6243-6254.View more citation formats
Fang W, Liu S, Nie Y. Anticancer Activity of Chamaejasmine: Effect on Tubulin Protein. Molecules. 2011; 16(8):6243-6254.Chicago/Turabian Style
Fang, Wenlong; Liu, Songtao; Nie, Yingkun. 2011. "Anticancer Activity of Chamaejasmine: Effect on Tubulin Protein." Molecules 16, no. 8: 6243-6254.