Next Article in Journal
Antioxidant and Cytotoxic Constituents from Wisteria sinensis
Previous Article in Journal
A New Pyranoxanthone from Calophyllum soulattri
Molecules 2011, 16(5), 4005-4019; doi:10.3390/molecules16054005
Article

Apigenin Isolated from the Medicinal Plant Elsholtzia rugulosa Prevents β-Amyloid 25–35-Induces Toxicity in Rat Cerebral Microvascular Endothelial Cells

1,* , 2,* , 2, 2, 1, 1, 1, 1 and 3,*
1 Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China 2 Shandong University Affiliated Jinan Central Hospital, Jinan 250013, China 3 Key Lab of Ministry of Education, National Research Center for Minority Medicine, Minzu University of China, Beijing 100081, China
* Authors to whom correspondence should be addressed.
Received: 28 March 2011 / Revised: 28 April 2011 / Accepted: 5 May 2011 / Published: 13 May 2011
(This article belongs to the Section Metabolites)
Download PDF [329 KB, uploaded 18 June 2014]

Abstract

Endothelial cells of cerebral capillaries forming the blood-brain barrier play an important role in the pathogenesis and therapy of Alzheimer’s disease. Amyloid-β peptides are key pathological elements in the development of this disease. Apigenin (4’,5,7-tetrahydroxyflavone) is a plant flavonoid and pharmacologically active agent that can be isolated from several plant species. In the present study, effects of apigenin obtained from the medicinal plant Elsholtzia rugulosa (Labiatae) on primary cultured rat cerebral microvascular endothelial cells (CMECs) mediated by amyloid-β peptide 25–35 (Aβ25–35) were examined. Aβ25–35 showed toxic effects on CMECs, involving reduction of cell viability, release of lactate dehydrogenase (LDH), increase of nuclear condensation, over-production of intracellular reactive oxygen species (ROS), decrease of superoxide dismutase (SOD) activity, and breakage of the barrier integrity and function. Based on this model, we demonstrated that apigenin from the medicinal plant Elsholtzia rugulosa protected cultured rat CMECs by increasing cell viability, reducing LDH release, relieving nuclear condensation, alleviating intracellular ROS generation, increasing SOD activity, and strengthening the barrier integrity through the preservation of transendothelial electrical resistance, permeability property and characteristic enzymatic activity after being exposed to Aβ25–35. In conclusion, apigenin isolated from Elsholtzia rugulosa has the ability to protect rat CMECs against Aβ25–35-induced toxicity.
Keywords: Alzheimer’s disease; amyloid-β peptide; apigenin; Elsholtzia rugulosa; microvascular endothelial cells Alzheimer’s disease; amyloid-β peptide; apigenin; Elsholtzia rugulosa; microvascular endothelial cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Zhao, L.; Hou, L.; Sun, H.; Yan, X.; Sun, X.; Li, J.; Bian, Y.; Chu, Y.; Liu, Q. Apigenin Isolated from the Medicinal Plant Elsholtzia rugulosa Prevents β-Amyloid 25–35-Induces Toxicity in Rat Cerebral Microvascular Endothelial Cells. Molecules 2011, 16, 4005-4019.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert