Molecules 2011, 16(5), 3885-3895; doi:10.3390/molecules16053885
Article

Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum

* email, * email, email, email, email and email
Received: 8 March 2011; in revised form: 30 April 2011 / Accepted: 5 May 2011 / Published: 9 May 2011
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The fungotoxicity against Botrytis cinerea of a flavonoid isolated from Pseudognaphalium robustum was analyzed. Two absorption column chromatographies and one semipreparative thin layer chromatography were used to purify the active flavonoid. It was determined, by 1H-NMR spectroscopy and co-elution with standards in HPLC, that this compound was 5,7-dihydroxy-3,8-dimethoxyflavone (gnaphaliin A). To determine the fungitoxicity of the purified compound, the effect on in vitro mycelial growth and conidial germination was studied. The compound concentration that reduced mycelial growth by 50% was 45.5 mg/mL. This compound also partially affected conidial germination of B. cinerea, reduced oxygen consumption by germinating conidia and affected the integrity of plasma membrane. Finally, using cyclic voltammetry, it was shown that the purified flavone had a pro-oxidant effect.
Keywords: antifungal activity; Botrytis cinerea; 5,7-dihydroxy-3,8-dimethoxyflavone; Pseudognaphalium robustum; pro-oxidants
PDF Full-text Download PDF Full-Text [273 KB, uploaded 18 June 2014 19:38 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Cotoras, M.; Mendoza, L.; Muñoz, A.; Yáñez, K.; Castro, P.; Aguirre, M. Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum. Molecules 2011, 16, 3885-3895.

AMA Style

Cotoras M, Mendoza L, Muñoz A, Yáñez K, Castro P, Aguirre M. Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum. Molecules. 2011; 16(5):3885-3895.

Chicago/Turabian Style

Cotoras, Milena; Mendoza, Leonora; Muñoz, Alexis; Yáñez, Karen; Castro, Paulo; Aguirre, María. 2011. "Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum." Molecules 16, no. 5: 3885-3895.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert