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Molecules 2011, 16(3), 2293-2303; doi:10.3390/molecules16032293
Article

Identification and Determination of Flavonoids in Astragali Radix by High Performance Liquid Chromatography Coupled with DAD and ESI-MS Detection

1, 1, 1, 1,* , 2 and 3
Received: 10 January 2011; in revised form: 24 February 2011 / Accepted: 4 March 2011 / Published: 9 March 2011
(This article belongs to the Section Metabolites)
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Abstract: A method for the analysis of flavonoids in Astragali Radix by high-performance liquid chromatography (HPLC) combined with photodiode-array detection (DAD) and an electrospray ionization (ESI) - mass spectrometry (MS) was developed. After the samples were extracted with ethanol, the optimum separation conditions for these analytes were achieved using a gradient elution system and a 2.0 × 150 mm Shimadzu VP-ODS column. Eight flavonoids were identified to exist in Astragali Radix based on their characteristic UV data and mass spectra. The concentrations of three major components in this herb—ononin, calycosin and formononetin—were determined by LC/ESI-MS in positive selective ion monitoring (SIM) mode. The calibration curves were linear in the range of 0.9~180.0 μg·mL−1 for ononin, 1.8~360.0 μg·mL−1 for calycosin and 1.4~280 μg·mL−1 for formononetin, respectively. The limits of quantification (LOQ) and detection (LOD) were 0.9 μg· mL−1 and 0.2 μg mL−1 for ononin, 1.8 μg mL−1 and 0.5 μg·mL−1 for calycosin, 1.4 μg mL−1 and 0.5 μg·mL−1 for formononetin, respectively. The standard recoveries were between 95.4~104.7%. The developed method was proven to be useful for the quantitative and qualitative analysis of flavonoid constituents in various resources of Astragali Radix.
Keywords: HPLC; DAD; ESI-MS; flavonoids; astragali radix HPLC; DAD; ESI-MS; flavonoids; astragali radix
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lv, Y.-W.; Hu, W.; Wang, Y.-L.; Huang, L.-F.; He, Y.-B.; Xie, X.-Z. Identification and Determination of Flavonoids in Astragali Radix by High Performance Liquid Chromatography Coupled with DAD and ESI-MS Detection. Molecules 2011, 16, 2293-2303.

AMA Style

Lv Y-W, Hu W, Wang Y-L, Huang L-F, He Y-B, Xie X-Z. Identification and Determination of Flavonoids in Astragali Radix by High Performance Liquid Chromatography Coupled with DAD and ESI-MS Detection. Molecules. 2011; 16(3):2293-2303.

Chicago/Turabian Style

Lv, Yan-Wen; Hu, Wei; Wang, Yu-Ling; Huang, Lan-Fang; He, Yun-Biao; Xie, Xian-Zhen. 2011. "Identification and Determination of Flavonoids in Astragali Radix by High Performance Liquid Chromatography Coupled with DAD and ESI-MS Detection." Molecules 16, no. 3: 2293-2303.



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