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Antioxidant Phenolic Compounds of Cassava (Manihot esculenta) from Hainan
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China
Hainan Key Laboratory for Research and Development of Natural Product from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Graduate School of Chinese Academy of Sciences, Yuquanlu 19A, Beijing 100049, China
Pharmacy Department of 187 Hospital PLA, Longkun South Road 100, Haikou 571159, China
* Authors to whom correspondence should be addressed.
Received: 24 October 2011; in revised form: 23 November 2011 / Accepted: 29 November 2011 / Published: 7 December 2011
Abstract: An activity-directed fractionation and purification process was used to isolate antioxidant components from cassava stems produced in Hainan. The ethyl acetate and n-butanol fractions showed greater DPPH˙and ABTS·+ scavenging activities than other fractions. The ethyl acetate fraction was subjected to column chromatography, to yield ten phenolic compounds: Coniferaldehyde (1), isovanillin (2), 6-deoxyjacareubin (3), scopoletin (4), syringaldehyde (5), pinoresinol (6), p-coumaric acid (7), ficusol (8), balanophonin (9) and ethamivan (10), which possess significant antioxidant activities. The relative order of DPPH· scavenging capacity for these compounds was ascorbic acid (reference) > 6 > 1 > 8 > 10 > 9 > 3 > 4 > 7 > 5 > 2, and that of ABTS·+ scavenging capacity was 5 > 7 > 1 > 10 > 4 > 6 > 8 > 2 > Trolox (reference compound) > 3 > 9. The results showed that these phenolic compounds contributed to the antioxidant activity of cassava.
Keywords: Manihot esculenta; isolation; phenolic compounds; DPPH; ABTS; antioxidant activity
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Yi, B.; Hu, L.; Mei, W.; Zhou, K.; Wang, H.; Luo, Y.; Wei, X.; Dai, H. Antioxidant Phenolic Compounds of Cassava (Manihot esculenta) from Hainan. Molecules 2011, 16, 10157-10167.
Yi B, Hu L, Mei W, Zhou K, Wang H, Luo Y, Wei X, Dai H. Antioxidant Phenolic Compounds of Cassava (Manihot esculenta) from Hainan. Molecules. 2011; 16(12):10157-10167.
Yi, Bo; Hu, Lifei; Mei, Wenli; Zhou, Kaibing; Wang, Hui; Luo, Ying; Wei, Xiaoyi; Dai, Haofu. 2011. "Antioxidant Phenolic Compounds of Cassava (Manihot esculenta) from Hainan." Molecules 16, no. 12: 10157-10167.