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Molecules 2011, 16(12), 10157-10167; doi:10.3390/molecules161210157
Article

Antioxidant Phenolic Compounds of Cassava (Manihot esculenta) from Hainan

1,3,4, 2, 2, 2, 2, 2, 1,*  and 2,*
1 Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China 2 Hainan Key Laboratory for Research and Development of Natural Product from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China 3 Graduate School of Chinese Academy of Sciences, Yuquanlu 19A, Beijing 100049, China 4 Pharmacy Department of 187 Hospital PLA, Longkun South Road 100, Haikou 571159, China
* Authors to whom correspondence should be addressed.
Received: 24 October 2011 / Revised: 23 November 2011 / Accepted: 29 November 2011 / Published: 7 December 2011
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Abstract

An activity-directed fractionation and purification process was used to isolate antioxidant components from cassava stems produced in Hainan. The ethyl acetate and n-butanol fractions showed greater DPPH˙and ABTS·+ scavenging activities than other fractions. The ethyl acetate fraction was subjected to column chromatography, to yield ten phenolic compounds: Coniferaldehyde (1), isovanillin (2), 6-deoxyjacareubin (3), scopoletin (4), syringaldehyde (5), pinoresinol (6), p-coumaric acid (7), ficusol (8), balanophonin (9) and ethamivan (10), which possess significant antioxidant activities. The relative order of DPPH· scavenging capacity for these compounds was ascorbic acid (reference) > 6 > 1 > 8 > 10 > 9 > 3 > 4 > 7 > 5 > 2, and that of ABTS·+ scavenging capacity was 5 > 7 > 1 > 10 > 4 > 6 > 8 > 2 > Trolox (reference compound) > 3 > 9. The results showed that these phenolic compounds contributed to the antioxidant activity of cassava.
Keywords: Manihot esculenta; isolation; phenolic compounds; DPPH; ABTS; antioxidant activity Manihot esculenta; isolation; phenolic compounds; DPPH; ABTS; antioxidant activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Yi, B.; Hu, L.; Mei, W.; Zhou, K.; Wang, H.; Luo, Y.; Wei, X.; Dai, H. Antioxidant Phenolic Compounds of Cassava (Manihot esculenta) from Hainan. Molecules 2011, 16, 10157-10167.

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