Abstract: An activity-directed fractionation and purification process was used to isolate antioxidant components from cassava stems produced in Hainan. The ethyl acetate and n-butanol fractions showed greater DPPH˙and ABTS·+ scavenging activities than other fractions. The ethyl acetate fraction was subjected to column chromatography, to yield ten phenolic compounds: Coniferaldehyde (1), isovanillin (2), 6-deoxyjacareubin (3), scopoletin (4), syringaldehyde (5), pinoresinol (6), p-coumaric acid (7), ficusol (8), balanophonin (9) and ethamivan (10), which possess significant antioxidant activities. The relative order of DPPH· scavenging capacity for these compounds was ascorbic acid (reference) > 6 > 1 > 8 > 10 > 9 > 3 > 4 > 7 > 5 > 2, and that of ABTS·+ scavenging capacity was 5 > 7 > 1 > 10 > 4 > 6 > 8 > 2 > Trolox (reference compound) > 3 > 9. The results showed that these phenolic compounds contributed to the antioxidant activity of cassava.
Keywords: Manihot esculenta; isolation; phenolic compounds; DPPH; ABTS; antioxidant activity
Export to BibTeX
MDPI and ACS Style
Yi, B.; Hu, L.; Mei, W.; Zhou, K.; Wang, H.; Luo, Y.; Wei, X.; Dai, H. Antioxidant Phenolic Compounds of Cassava (Manihot esculenta) from Hainan. Molecules 2011, 16, 10157-10167.
Yi B, Hu L, Mei W, Zhou K, Wang H, Luo Y, Wei X, Dai H. Antioxidant Phenolic Compounds of Cassava (Manihot esculenta) from Hainan. Molecules. 2011; 16(12):10157-10167.
Yi, Bo; Hu, Lifei; Mei, Wenli; Zhou, Kaibing; Wang, Hui; Luo, Ying; Wei, Xiaoyi; Dai, Haofu. 2011. "Antioxidant Phenolic Compounds of Cassava (Manihot esculenta) from Hainan." Molecules 16, no. 12: 10157-10167.