Chamaejasmine Induces Apoptosis in Human Lung Adenocarcinoma A549 Cells through a Ros-Mediated Mitochondrial Pathway

doi:10.3390/molecules16108165

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Molecules 2011, 16(10), 8165-8180; doi:10.3390/molecules16108165

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Chamaejasmine Induces Apoptosis in Human Lung Adenocarcinoma A549 Cells through a Ros-Mediated Mitochondrial Pathway

Hongyang Yu^1,2,†, Tingting Zhang^3,†, Li Cai³, Yuanyuan Qu², Songliu Hu², Guanglu Dong¹, Rongwei Guan⁵, Xiangying Xu^2,4,* and Lina Xing^1,*

¹ Department of Radiation Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China ² Department of Radiation Oncology, The Third Affiliated (Tumor) Hospital, Harbin Medical University, Harbin 150040, China ³ Oncology Department of Internal Medicine, The Third Affiliated (Tumor) Hospital, Harbin Medical University, Harbin 150040, China ⁴ Harbin Medical University Cancer Research Institute, Harbin 150040, China ⁵ Key Laboratory of Medical Genetics (Harbin Medical University), Heilongjiang Higher Education Institutions, Harbin 150081, China ^† These authors contributed equally to this work.

* Authors to whom correspondence should be addressed.

Received: 19 August 2011; in revised form: 21 September 2011 / Accepted: 23 September 2011 / Published: 27 September 2011

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Abstract: In the present study, the anticancer activity of chamaejasmine towards A549 human lung adenocarcinoma cells was investigated. In order to explore the underlying mechanism of cell growth inhibition of chamaejasmine, cell cycle distribution, ROS generation, mitochondrial membrane potential (Δψ_m) disruption, and expression of cytochrome c, Bax, Bcl-2, caspase-3, caspase-9 and PARP were measured in A549 cells. Chamaejasmine inhibited the growth of A549 cells in a time and dose-dependent manner. The IC₅₀ value was 7.72 µM after 72 h treatment. Chamaejasmine arrested the cell cycle in the G2/M phase and induced apoptosis via a ROS-mediated mitochondria-dependent pathway. Western blot analysis showed that chamaejasmine inhibited Bcl-2 expression and induced Bax expression to desintegrate the outer mitochondrial membrane and causing cytochrome c release. Mitochondrial cytochrome c release was associated with the activation of caspase-9 and caspase-3 cascade, and active-caspase-3 was involved in PARP cleavage. All of these signal transduction pathways are involved in initiating apoptosis. To the best of our knowledge, this is the first report demonstrating the cytotoxic activity of chamaejasmine towards A549 in vitro.

Keywords: chamaejasmine; A549; Apoptosis; ROS; mitochondrial

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Cite This Article

MDPI and ACS Style

Yu, H.; Zhang, T.; Cai, L.; Qu, Y.; Hu, S.; Dong, G.; Guan, R.; Xu, X.; Xing, L. Chamaejasmine Induces Apoptosis in Human Lung Adenocarcinoma A549 Cells through a Ros-Mediated Mitochondrial Pathway. Molecules 2011, 16, 8165-8180.

AMA Style

Yu H, Zhang T, Cai L, Qu Y, Hu S, Dong G, Guan R, Xu X, Xing L. Chamaejasmine Induces Apoptosis in Human Lung Adenocarcinoma A549 Cells through a Ros-Mediated Mitochondrial Pathway. Molecules. 2011; 16(10):8165-8180.

Chicago/Turabian Style

Yu, Hongyang; Zhang, Tingting; Cai, Li; Qu, Yuanyuan; Hu, Songliu; Dong, Guanglu; Guan, Rongwei; Xu, Xiangying; Xing, Lina. 2011. "Chamaejasmine Induces Apoptosis in Human Lung Adenocarcinoma A549 Cells through a Ros-Mediated Mitochondrial Pathway." Molecules 16, no. 10: 8165-8180.

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