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Chamaejasmine Induces Apoptosis in Human Lung Adenocarcinoma A549 Cells through a Ros-Mediated Mitochondrial Pathway
Department of Radiation Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China
Department of Radiation Oncology, The Third Affiliated (Tumor) Hospital, Harbin Medical University, Harbin 150040, China
Oncology Department of Internal Medicine, The Third Affiliated (Tumor) Hospital, Harbin Medical University, Harbin 150040, China
Harbin Medical University Cancer Research Institute, Harbin 150040, China
Key Laboratory of Medical Genetics (Harbin Medical University), Heilongjiang Higher Education Institutions, Harbin 150081, China
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 19 August 2011; in revised form: 21 September 2011 / Accepted: 23 September 2011 / Published: 27 September 2011
Abstract: In the present study, the anticancer activity of chamaejasmine towards A549 human lung adenocarcinoma cells was investigated. In order to explore the underlying mechanism of cell growth inhibition of chamaejasmine, cell cycle distribution, ROS generation, mitochondrial membrane potential (Δψm) disruption, and expression of cytochrome c, Bax, Bcl-2, caspase-3, caspase-9 and PARP were measured in A549 cells. Chamaejasmine inhibited the growth of A549 cells in a time and dose-dependent manner. The IC50 value was 7.72 µM after 72 h treatment. Chamaejasmine arrested the cell cycle in the G2/M phase and induced apoptosis via a ROS-mediated mitochondria-dependent pathway. Western blot analysis showed that chamaejasmine inhibited Bcl-2 expression and induced Bax expression to desintegrate the outer mitochondrial membrane and causing cytochrome c release. Mitochondrial cytochrome c release was associated with the activation of caspase-9 and caspase-3 cascade, and active-caspase-3 was involved in PARP cleavage. All of these signal transduction pathways are involved in initiating apoptosis. To the best of our knowledge, this is the first report demonstrating the cytotoxic activity of chamaejasmine towards A549 in vitro.
Keywords: chamaejasmine; A549; Apoptosis; ROS; mitochondrial
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MDPI and ACS Style
Yu, H.; Zhang, T.; Cai, L.; Qu, Y.; Hu, S.; Dong, G.; Guan, R.; Xu, X.; Xing, L. Chamaejasmine Induces Apoptosis in Human Lung Adenocarcinoma A549 Cells through a Ros-Mediated Mitochondrial Pathway. Molecules 2011, 16, 8165-8180.
Yu H, Zhang T, Cai L, Qu Y, Hu S, Dong G, Guan R, Xu X, Xing L. Chamaejasmine Induces Apoptosis in Human Lung Adenocarcinoma A549 Cells through a Ros-Mediated Mitochondrial Pathway. Molecules. 2011; 16(10):8165-8180.
Yu, Hongyang; Zhang, Tingting; Cai, Li; Qu, Yuanyuan; Hu, Songliu; Dong, Guanglu; Guan, Rongwei; Xu, Xiangying; Xing, Lina. 2011. "Chamaejasmine Induces Apoptosis in Human Lung Adenocarcinoma A549 Cells through a Ros-Mediated Mitochondrial Pathway." Molecules 16, no. 10: 8165-8180.