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Molecules 2011, 16(1), 532-542; doi:10.3390/molecules16010532
Article

White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase

1, 1, 2, 1, 1, 1, 3,†,*  and 1,*
Received: 16 November 2010; in revised form: 19 December 2010 / Accepted: 11 January 2011 / Published: 12 January 2011
(This article belongs to the Special Issue Enzyme-Catalyzed Reactions)
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Abstract: White spot syndrome virus (WSSV) is the causative agent of white spot syndrome, one of the most devastating diseases in shrimp aquaculture. The genome of WSSV includes a gene that encodes a putative family B DNA polymerase (ORF514), which is 16% identical in amino acid sequence to the Herpes virus 1 DNA polymerase. The aim of this work was to demonstrate the activity of the WSSV ORF514-encoded protein as a DNA polymerase and hence a putative antiviral target. A 3.5 kbp fragment encoding the conserved polymerase and exonuclease domains of ORF514 was overexpressed in bacteria. The recombinant protein showed polymerase activity but with very low level of processivity. Molecular modeling of the catalytic protein core encoded in ORF514 revealed a canonical polymerase fold. Amino acid sequence alignments of ORF514 indicate the presence of a putative PIP box, suggesting that the encoded putative DNA polymerase may use a host processivity factor for optimal activity. We postulate that WSSV ORF514 encodes a bona fide DNA polymerase that requires accessory proteins for activity and maybe target for drugs or compounds that inhibit viral DNA replication.
Keywords: DNA polymerase family B; WSSV; white spot syndrome virus; polymerase activity; Crustacea; shrimp; prawn; Litopenaeus vannamei; Decapoda; invertebrate DNA polymerase family B; WSSV; white spot syndrome virus; polymerase activity; Crustacea; shrimp; prawn; Litopenaeus vannamei; Decapoda; invertebrate
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

De-la-Re-Vega, E.; Garcia-Orozco, K.D.; Arvizu-Flores, A.A.; Yepiz-Plascencia, G.; Muhlia-Almazan, A.; Hernández, J.; Brieba, L.G.; Sotelo-Mundo, R.R. White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase. Molecules 2011, 16, 532-542.

AMA Style

De-la-Re-Vega E, Garcia-Orozco KD, Arvizu-Flores AA, Yepiz-Plascencia G, Muhlia-Almazan A, Hernández J, Brieba LG, Sotelo-Mundo RR. White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase. Molecules. 2011; 16(1):532-542.

Chicago/Turabian Style

De-la-Re-Vega, Enrique; Garcia-Orozco, Karina D.; Arvizu-Flores, Aldo A.; Yepiz-Plascencia, Gloria; Muhlia-Almazan, Adriana; Hernández, Jesús; Brieba, Luis G.; Sotelo-Mundo, Rogerio R. 2011. "White Spot Syndrome Virus Orf514 Encodes a Bona Fide DNA Polymerase." Molecules 16, no. 1: 532-542.



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