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Molecules 2010, 15(8), 5818-5830; doi:10.3390/molecules15085818
Article

High Molecular Weight Glucan of the Culinary Medicinal Mushroom Agaricus bisporus is an α-Glucan that Forms Complexes with Low Molecular Weight Galactan

1,2, 2, 1, 2, 3 and 1,*
Received: 16 July 2010; in revised form: 20 August 2010 / Accepted: 24 August 2010 / Published: 25 August 2010
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Abstract: An a-glucan was isolated from the culinary medicinal mushroom A. bisporus by hot water extraction, ethanol precipitation and DEAE-cellulose chromatography. The resulting material showed a single HMW peak excluded from a Sephadex G50 column that could completely be degraded by α-amylase treatment. After heating in 1% SDS a small additional peak of low MW eluted from the G50 column. The monosaccharide composition of the main peak was evaluated by HPLC, and was found to consist of a majority of glucose (97.6%), and a minor proportion of galactose (2.4%). Methylation analysis and degradation by a-amylase indicated the presence of an a-glucan with a main chain consisting of (1®4)-linked units, substituted at O-6 by α-D-glucopyranose single-units in the relation 1:8. Mono- (13C-, 1H-NMR) and bidimensional [1H (obs.),13C-HSQC] spectroscopy analysis confirmed the a-configuration of the Glcp residues by low frequency resonances of C-1 at d 100.6, 100.2, and 98.8 ppm and H-1 high field ones at d 5.06, 5.11, and 4.74 ppm. The DEPT-13C-NMR allowed assigning the non-substituted and O-substituted –CH2 signals at d 60.3/60.8 and 66.2 ppm, respectively. Other assignments were attributed to C-2, C-3, C-4, C-5 and C-6 of the non-reducing ends at d 71.8; 72.8; 70.0; 71.3 and 60.3/60.8 ppm, respectively. The minor proportion of galactose that was demonstrated was probably derived from a complex between the a-glucan and a low molecular weight galactan.
Keywords: Agaricus bisporus; basidiomycetes; a-glucan; medicinal mushrooms; polysaccharides; NMR Agaricus bisporus; basidiomycetes; a-glucan; medicinal mushrooms; polysaccharides; NMR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Smiderle, F.R.; Sassaki, G.L.; Arkel, J.; Iacomini, M.; Wichers, H.J.; Griensven, L.J.V. High Molecular Weight Glucan of the Culinary Medicinal Mushroom Agaricus bisporus is an α-Glucan that Forms Complexes with Low Molecular Weight Galactan. Molecules 2010, 15, 5818-5830.

AMA Style

Smiderle FR, Sassaki GL, Arkel J, Iacomini M, Wichers HJ, Griensven LJV. High Molecular Weight Glucan of the Culinary Medicinal Mushroom Agaricus bisporus is an α-Glucan that Forms Complexes with Low Molecular Weight Galactan. Molecules. 2010; 15(8):5818-5830.

Chicago/Turabian Style

Smiderle, Fhernanda R.; Sassaki, Guilherme L.; Arkel, Jeroen van; Iacomini, Marcello; Wichers, Harry J.; Griensven, Leo J.L.D. Van. 2010. "High Molecular Weight Glucan of the Culinary Medicinal Mushroom Agaricus bisporus is an α-Glucan that Forms Complexes with Low Molecular Weight Galactan." Molecules 15, no. 8: 5818-5830.


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