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Molecules 2010, 15(8), 5742-5755; doi:10.3390/molecules15085742
Article

Development of a Sphingosylphosphorylcholine Detection System Using RNA Aptamers

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1 VALWAY Technology Center, NEC Soft, Ltd., Tokyo 136-8627, Japan 2 Biotechnology Research Group, Fundamental Research Department, Fujirebio Inc., Tokyo, 192-0031, Japan
* Author to whom correspondence should be addressed.
Received: 27 July 2010 / Revised: 16 August 2010 / Accepted: 17 August 2010 / Published: 20 August 2010
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Abstract

Sphingosylphosphorylcholine (SPC) is a lysosphingolipid that exerts multiple functions, including acting as a spasmogen, as a mitogenic factor for various types of cells, and sometimes as an inflammatory mediator. Currently, liquid chromatography/tandem mass spectrometry (LC/MS/MS) is used for the quantitation of SPC. However, because of the complicated procedures required it may not be cost effective, hampering its regular usage in a routine practical SPC monitoring. In this report, we have generated RNA aptamers that bind to SPC with high affinity using an in vitro selection procedure and developed an enzyme-linked aptamer assay system using the minimized SPC aptamer that can successfully distinguish SPC from the structurally related sphingosine 1-phosphate (S1P). This is the first case of the Systematic Evolution of Ligands by EXponential enrichment (SELEX) process being performed with a lysosphingolipid. The SPC aptamers would be valuable tools for the development of aptamer-based medical diagnosis and for elucidating the biological role of SPC.
Keywords: aptamer; enzyme-linked aptamer assay; sphingosine 1-phosphate; sphingosylphosphorylcholine; systematic evolution of ligands by exponential enrichment aptamer; enzyme-linked aptamer assay; sphingosine 1-phosphate; sphingosylphosphorylcholine; systematic evolution of ligands by exponential enrichment
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Horii, K.; Omi, K.; Yoshida, Y.; Imai, Y.; Sakai, N.; Oka, A.; Masuda, H.; Furuichi, M.; Tanimoto, T.; Waga, I. Development of a Sphingosylphosphorylcholine Detection System Using RNA Aptamers. Molecules 2010, 15, 5742-5755.

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