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Molecules 2010, 15(8), 5742-5755; doi:10.3390/molecules15085742
Article

Development of a Sphingosylphosphorylcholine Detection System Using RNA Aptamers

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Received: 27 July 2010; in revised form: 16 August 2010 / Accepted: 17 August 2010 / Published: 20 August 2010
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Abstract: Sphingosylphosphorylcholine (SPC) is a lysosphingolipid that exerts multiple functions, including acting as a spasmogen, as a mitogenic factor for various types of cells, and sometimes as an inflammatory mediator. Currently, liquid chromatography/tandem mass spectrometry (LC/MS/MS) is used for the quantitation of SPC. However, because of the complicated procedures required it may not be cost effective, hampering its regular usage in a routine practical SPC monitoring. In this report, we have generated RNA aptamers that bind to SPC with high affinity using an in vitro selection procedure and developed an enzyme-linked aptamer assay system using the minimized SPC aptamer that can successfully distinguish SPC from the structurally related sphingosine 1-phosphate (S1P). This is the first case of the Systematic Evolution of Ligands by EXponential enrichment (SELEX) process being performed with a lysosphingolipid. The SPC aptamers would be valuable tools for the development of aptamer-based medical diagnosis and for elucidating the biological role of SPC.
Keywords: aptamer; enzyme-linked aptamer assay; sphingosine 1-phosphate; sphingosylphosphorylcholine; systematic evolution of ligands by exponential enrichment aptamer; enzyme-linked aptamer assay; sphingosine 1-phosphate; sphingosylphosphorylcholine; systematic evolution of ligands by exponential enrichment
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Horii, K.; Omi, K.; Yoshida, Y.; Imai, Y.; Sakai, N.; Oka, A.; Masuda, H.; Furuichi, M.; Tanimoto, T.; Waga, I. Development of a Sphingosylphosphorylcholine Detection System Using RNA Aptamers. Molecules 2010, 15, 5742-5755.

AMA Style

Horii K, Omi K, Yoshida Y, Imai Y, Sakai N, Oka A, Masuda H, Furuichi M, Tanimoto T, Waga I. Development of a Sphingosylphosphorylcholine Detection System Using RNA Aptamers. Molecules. 2010; 15(8):5742-5755.

Chicago/Turabian Style

Horii, Katsunori; Omi, Kazuya; Yoshida, Yoshihito; Imai, Yuka; Sakai, Nobuya; Oka, Asako; Masuda, Hiromi; Furuichi, Makio; Tanimoto, Tetsuji; Waga, Iwao. 2010. "Development of a Sphingosylphosphorylcholine Detection System Using RNA Aptamers." Molecules 15, no. 8: 5742-5755.


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