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Molecules 2010, 15(8), 5692-5707; doi:10.3390/molecules15085692
Article

Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties

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Received: 28 May 2010; in revised form: 28 July 2010 / Accepted: 5 August 2010 / Published: 18 August 2010
(This article belongs to the Special Issue Solid Phase Synthesis)
Download PDF [253 KB, uploaded 18 June 2014]
Abstract: An efficient route for the synthesis of the phosphoramidite derivative of 5-methylcytosine bearing a tert-butylsulfanyl group protected thiol is described. This building block is used for the preparation of oligonucleotides carrying a thiol group at the nucleobase at the internal position of a DNA sequence. The resulting thiolated oligonucleotides are useful intermediates to generate oligonucleotide conjugates carrying molecules of interest at internal positions of a DNA sequence.
Keywords: solid-phase synthesis; oligonucleotides; DNA; thiol; oligonucleotide conjugates solid-phase synthesis; oligonucleotides; DNA; thiol; oligonucleotide conjugates
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Pérez-Rentero, S.; Garibotti, A.V.; Eritja, R. Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties. Molecules 2010, 15, 5692-5707.

AMA Style

Pérez-Rentero S, Garibotti AV, Eritja R. Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties. Molecules. 2010; 15(8):5692-5707.

Chicago/Turabian Style

Pérez-Rentero, Sónia; Garibotti, Alejandra V.; Eritja, Ramón. 2010. "Solid-Phase Synthesis of Oligodeoxynucleotides Containing N4-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties." Molecules 15, no. 8: 5692-5707.


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