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Molecules 2010, 15(8), 5525-5546; doi:10.3390/molecules15085525
Review

Nanofabrication of Nonfouling Surfaces for Micropatterning of Cell and Microtissue

Received: 11 June 2010; in revised form: 20 July 2010 / Accepted: 4 August 2010 / Published: 10 August 2010
(This article belongs to the Special Issue High-throughput Screening)
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Abstract: Surface engineering techniques for cellular micropatterning are emerging as important tools to clarify the effects of the microenvironment on cellular behavior, as cells usually integrate and respond the microscale environment, such as chemical and mechanical properties of the surrounding fluid and extracellular matrix, soluble protein factors, small signal molecules, and contacts with neighboring cells. Furthermore, recent progress in cellular micropatterning has contributed to the development of cell-based biosensors for the functional characterization and detection of drugs, pathogens, toxicants, and odorants. In this regards, the ability to control shape and spreading of attached cells and cell-cell contacts through the form and dimension of the cell-adhesive patches with high precision is important. Commitment of stem cells to different specific lineages depends strongly on cell shape, implying that controlled microenvironments through engineered surfaces may not only be a valuable approach towards fundamental cell-biological studies, but also of great importance for the design of cell culture substrates for tissue engineering. To develop this kind of cellular microarray composed of a cell-resistant surface and cell attachment region, micropatterning a protein-repellent surface is important because cellular adhesion and proliferation are regulated by protein adsorption. The focus of this review is on the surface engineering aspects of biologically motivated micropatterning of two-dimensional surfaces with the aim to provide an introductory overview described in the literature. In particular, the importance of non-fouling surface chemistries is discussed.
Keywords: micropatterning; spheroid; co-culture; 3D culture; non-fouling surface; cell-cell interactions micropatterning; spheroid; co-culture; 3D culture; non-fouling surface; cell-cell interactions
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Otsuka, H. Nanofabrication of Nonfouling Surfaces for Micropatterning of Cell and Microtissue. Molecules 2010, 15, 5525-5546.

AMA Style

Otsuka H. Nanofabrication of Nonfouling Surfaces for Micropatterning of Cell and Microtissue. Molecules. 2010; 15(8):5525-5546.

Chicago/Turabian Style

Otsuka, Hidenori. 2010. "Nanofabrication of Nonfouling Surfaces for Micropatterning of Cell and Microtissue." Molecules 15, no. 8: 5525-5546.



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