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Molecules 2010, 15(5), 2935-2948; doi:10.3390/molecules15052935
Article

Artificial Self-Sufficient P450 in Reversed Micelles

1,2
, 4
, 5
 and 1,2,3,*
Received: 11 March 2010; in revised form: 14 April 2010 / Accepted: 23 April 2010 / Published: 27 April 2010
(This article belongs to the Special Issue Macromolecules: Chemistry, Medicinal and Functional Materials)
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Abstract: Cytochrome P450s are heme-containing monooxygenases that require electron transfer proteins for their catalytic activities. They prefer hydrophobic compounds as substrates and it is, therefore, desirable to perform their reactions in non-aqueous media. Reversed micelles can stably encapsulate proteins in nano-scaled water pools in organic solvents. However, in the reversed micellar system, when multiple proteins are involved in a reaction they can be separated into different micelles and it is then difficult to transfer electrons between proteins. We show here that an artificial self-sufficient cytochrome P450, which is an enzymatically crosslinked fusion protein composed of P450 and electron transfer proteins, showed micelle-size dependent catalytic activity in a reversed micellar system. Furthermore, the presence of thermostable alcohol dehydrogenase promoted the P450-catalyzed reaction due to cofactor regeneration.
Keywords: artificial self-sufficient cytochrome P450; cofactor regeneration; transglutaminase; reversed micelles artificial self-sufficient cytochrome P450; cofactor regeneration; transglutaminase; reversed micelles
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hirakawa, H.; Kamiya, N.; Kawarabayasi, Y.; Nagamune, T. Artificial Self-Sufficient P450 in Reversed Micelles. Molecules 2010, 15, 2935-2948.

AMA Style

Hirakawa H, Kamiya N, Kawarabayasi Y, Nagamune T. Artificial Self-Sufficient P450 in Reversed Micelles. Molecules. 2010; 15(5):2935-2948.

Chicago/Turabian Style

Hirakawa, Hidehiko; Kamiya, Noriho; Kawarabayasi, Yutaka; Nagamune, Teruyuki. 2010. "Artificial Self-Sufficient P450 in Reversed Micelles." Molecules 15, no. 5: 2935-2948.



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