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Artificial Self-Sufficient P450 in Reversed Micelles
Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Center for NanoBio Integration, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Department of Applied Chemistry, Graduate School of Engineering & Center for Future Chemistry, Kyushu University, Motooka 744, Fukuoka 819-0395, Japan
New Energy and Industrial Technology Development Organization, 19F Muza Kawasaki Building, 1310, Omiya-cho, Saiwai-ku, Kawasaki City Kanagawa 212-8554, Japan
* Author to whom correspondence should be addressed.
Received: 11 March 2010; in revised form: 14 April 2010 / Accepted: 23 April 2010 / Published: 27 April 2010
Abstract: Cytochrome P450s are heme-containing monooxygenases that require electron transfer proteins for their catalytic activities. They prefer hydrophobic compounds as substrates and it is, therefore, desirable to perform their reactions in non-aqueous media. Reversed micelles can stably encapsulate proteins in nano-scaled water pools in organic solvents. However, in the reversed micellar system, when multiple proteins are involved in a reaction they can be separated into different micelles and it is then difficult to transfer electrons between proteins. We show here that an artificial self-sufficient cytochrome P450, which is an enzymatically crosslinked fusion protein composed of P450 and electron transfer proteins, showed micelle-size dependent catalytic activity in a reversed micellar system. Furthermore, the presence of thermostable alcohol dehydrogenase promoted the P450-catalyzed reaction due to cofactor regeneration.
Keywords: artificial self-sufficient cytochrome P450; cofactor regeneration; transglutaminase; reversed micelles
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Cite This Article
MDPI and ACS Style
Hirakawa, H.; Kamiya, N.; Kawarabayasi, Y.; Nagamune, T. Artificial Self-Sufficient P450 in Reversed Micelles. Molecules 2010, 15, 2935-2948.
Hirakawa H, Kamiya N, Kawarabayasi Y, Nagamune T. Artificial Self-Sufficient P450 in Reversed Micelles. Molecules. 2010; 15(5):2935-2948.
Hirakawa, Hidehiko; Kamiya, Noriho; Kawarabayasi, Yutaka; Nagamune, Teruyuki. 2010. "Artificial Self-Sufficient P450 in Reversed Micelles." Molecules 15, no. 5: 2935-2948.