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Molecules 2010, 15(12), 9145-9156; doi:10.3390/molecules15129145
Article

Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice

1
,
1,2,* , 3
,
2
 and
2
1 Biotechnology Laboratory of Chinese Medicine, Macau University of Science and Technology, Macau, China 2 Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China 3 Analysis Center, Rudong County Grain Bureau, Nantong, 226400, Jiangsu, China
* Author to whom correspondence should be addressed.
Received: 19 September 2010 / Revised: 22 November 2010 / Accepted: 8 December 2010 / Published: 10 December 2010
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Abstract

Prunella vulgaris L. (PV, Labiatae) is known as a self-heal herb. The different extracts of dried spikes were studied for the best antioxidant active compounds. The 60% ethanol extract (P-60) showed strong antioxidant activity based on the results of 2,2’-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS˙+), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay methods. High performance liquid chromatography (HPLC) and LC/MS analysis showed that the main active compounds in P-60 were phenols, such as caffeic acid, rosmarinic acid, rutin and quercetin. Total phenols were highly correlated with the antioxidant activity (R2 = 0.9988 in ABTS˙+; 0.6284 in DPPH and 0.9673 FRAP tests). P-60 could inhibit significantly the tumor growth in C57BL/6 mice. It can also been showed that increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) content in serum of tumor-bearing mice. These results suggested that P-60 extract had high antioxidant activity in vitro and in vivo and total phenols played an important role in antioxidant activity for inhibition of tumor growth.
Keywords: Prunella vulgaris L; total phenols; antioxidant activity; HPLC-DAD; SOD; MDA; tumor-bearing mice Prunella vulgaris L; total phenols; antioxidant activity; HPLC-DAD; SOD; MDA; tumor-bearing mice
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Feng, L.; Jia, X.; Zhu, M.-M.; Chen, Y.; Shi, F. Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice. Molecules 2010, 15, 9145-9156.

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