Abstract: Prunella vulgaris L. (PV, Labiatae) is known as a self-heal herb. The different extracts of dried spikes were studied for the best antioxidant active compounds. The 60% ethanol extract (P-60) showed strong antioxidant activity based on the results of 2,2’-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS˙+), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay methods. High performance liquid chromatography (HPLC) and LC/MS analysis showed that the main active compounds in P-60 were phenols, such as caffeic acid, rosmarinic acid, rutin and quercetin. Total phenols were highly correlated with the antioxidant activity (R2 = 0.9988 in ABTS˙+; 0.6284 in DPPH and 0.9673 FRAP tests). P-60 could inhibit significantly the tumor growth in C57BL/6 mice. It can also been showed that increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) content in serum of tumor-bearing mice. These results suggested that P-60 extract had high antioxidant activity in vitro and in vivo and total phenols played an important role in antioxidant activity for inhibition of tumor growth.
Keywords: Prunella vulgaris L; total phenols; antioxidant activity; HPLC-DAD; SOD; MDA; tumor-bearing mice
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Feng, L.; Jia, X.; Zhu, M.-M.; Chen, Y.; Shi, F. Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice. Molecules 2010, 15, 9145-9156.
Feng L, Jia X, Zhu M-M, Chen Y, Shi F. Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice. Molecules. 2010; 15(12):9145-9156.
Feng, Liang; Jia, Xiaobin; Zhu, Mao-Mao; Chen, Yan; Shi, Feng. 2010. "Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice." Molecules 15, no. 12: 9145-9156.