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Molecules 2010, 15(12), 9145-9156; doi:10.3390/molecules15129145
Article

Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice

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Received: 19 September 2010; in revised form: 22 November 2010 / Accepted: 8 December 2010 / Published: 10 December 2010
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Abstract: Prunella vulgaris L. (PV, Labiatae) is known as a self-heal herb. The different extracts of dried spikes were studied for the best antioxidant active compounds. The 60% ethanol extract (P-60) showed strong antioxidant activity based on the results of 2,2’-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS˙+), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay methods. High performance liquid chromatography (HPLC) and LC/MS analysis showed that the main active compounds in P-60 were phenols, such as caffeic acid, rosmarinic acid, rutin and quercetin. Total phenols were highly correlated with the antioxidant activity (R2 = 0.9988 in ABTS˙+; 0.6284 in DPPH and 0.9673 FRAP tests). P-60 could inhibit significantly the tumor growth in C57BL/6 mice. It can also been showed that increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) content in serum of tumor-bearing mice. These results suggested that P-60 extract had high antioxidant activity in vitro and in vivo and total phenols played an important role in antioxidant activity for inhibition of tumor growth.
Keywords: Prunella vulgaris L; total phenols; antioxidant activity; HPLC-DAD; SOD; MDA; tumor-bearing mice Prunella vulgaris L; total phenols; antioxidant activity; HPLC-DAD; SOD; MDA; tumor-bearing mice
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Feng, L.; Jia, X.; Zhu, M.-M.; Chen, Y.; Shi, F. Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice. Molecules 2010, 15, 9145-9156.

AMA Style

Feng L, Jia X, Zhu M-M, Chen Y, Shi F. Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice. Molecules. 2010; 15(12):9145-9156.

Chicago/Turabian Style

Feng, Liang; Jia, Xiaobin; Zhu, Mao-Mao; Chen, Yan; Shi, Feng. 2010. "Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice." Molecules 15, no. 12: 9145-9156.



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