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Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice
Biotechnology Laboratory of Chinese Medicine, Macau University of Science and Technology, Macau, China
Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China
Analysis Center, Rudong County Grain Bureau, Nantong, 226400, Jiangsu, China
* Author to whom correspondence should be addressed.
Received: 19 September 2010; in revised form: 22 November 2010 / Accepted: 8 December 2010 / Published: 10 December 2010
Abstract: Prunella vulgaris L. (PV, Labiatae) is known as a self-heal herb. The different extracts of dried spikes were studied for the best antioxidant active compounds. The 60% ethanol extract (P-60) showed strong antioxidant activity based on the results of 2,2’-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS˙+), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay methods. High performance liquid chromatography (HPLC) and LC/MS analysis showed that the main active compounds in P-60 were phenols, such as caffeic acid, rosmarinic acid, rutin and quercetin. Total phenols were highly correlated with the antioxidant activity (R2 = 0.9988 in ABTS˙+; 0.6284 in DPPH and 0.9673 FRAP tests). P-60 could inhibit significantly the tumor growth in C57BL/6 mice. It can also been showed that increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) content in serum of tumor-bearing mice. These results suggested that P-60 extract had high antioxidant activity in vitro and in vivo and total phenols played an important role in antioxidant activity for inhibition of tumor growth.
Keywords: Prunella vulgaris L; total phenols; antioxidant activity; HPLC-DAD; SOD; MDA; tumor-bearing mice
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Cite This Article
MDPI and ACS Style
Feng, L.; Jia, X.; Zhu, M.-M.; Chen, Y.; Shi, F. Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice. Molecules 2010, 15, 9145-9156.
Feng L, Jia X, Zhu M-M, Chen Y, Shi F. Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice. Molecules. 2010; 15(12):9145-9156.
Feng, Liang; Jia, Xiaobin; Zhu, Mao-Mao; Chen, Yan; Shi, Feng. 2010. "Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice." Molecules 15, no. 12: 9145-9156.