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Molecules 2010, 15(12), 9092-9103; doi:10.3390/molecules15129092

Structural Relationship and Binding Mechanisms of Five Flavonoids with Bovine Serum Albumin

1,2, 1 and 1,*
1 Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China 2 Department of Pharmacognosy, College of Pharmacy, 3rd Military Medical University, Chongqing, China
* Author to whom correspondence should be addressed.
Received: 9 October 2010 / Revised: 1 December 2010 / Accepted: 6 December 2010 / Published: 9 December 2010
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Flavonoids are structurally diverse and the most ubiquitous groups of dietary polyphenols distributed in various fruits and vegetables. In this study, the interaction between five flavonoids, namely formononetin-7-O-β-D-glucoside, calycosin- 7-O-β-D-glucoside, calycosin, rutin, and quercetin, and bovine serum albumin (BSA) was investigated by fluorescence and UV-vis absorbance spectroscopy. In the discussion, it was proved that the fluorescence quenching of BSA by flavonoids was a result of the formation of a flavonoid-BSA complex. Fluorescence quenching constants were determined using the Stern-Volmer and Lineweaver-Burk equations to provide a measure of the binding affinity between the flavonoids and BSA. The binding constants ranked in the order quercetin > rutin > calycosin > calycosin-7-O-β-D-glucoside ≈ formononetin-7-O-β-D-glucoside. The results of thermodynamic parameters ΔG, ΔH, and ΔS at different temperatures indicated that the hydrophobic interaction played a major role in flavonoid-BSA association. The distance r between BSA and acceptor flavonoids was also obtained according to Förster’s theory of non-radiative energy transfer.
Keywords: flavonoids; bovine serum albumin; fluorescence quenching; binding mechanism flavonoids; bovine serum albumin; fluorescence quenching; binding mechanism
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Liu, E.-H.; Qi, L.-W.; Li, P. Structural Relationship and Binding Mechanisms of Five Flavonoids with Bovine Serum Albumin. Molecules 2010, 15, 9092-9103.

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