Molecules 2009, 14(9), 3509-3527; doi:10.3390/molecules14093509
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Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development

Bradley A. Carlson 1,* email, Min-Hyuk Yoo 1 email, Petra A. Tsuji 1,2,3 email, Vadim N. Gladyshev 4 email and Dolph L. Hatfield 1 email
1 Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA 2 Cancer Prevention Fellowship Program, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA 3 Nutritional Science Research Group, Division of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA 4 Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, NE 68588, USA
* Author to whom correspondence should be addressed.
Received: 17 August 2009; in revised form: 3 September 2009 / Accepted: 7 September 2009 / Published: 10 September 2009
(This article belongs to the Special Issue Selenium and Tellurium Chemistry)
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Abstract: Selenium (Se) deficiency has been known for many years to be associated with disease, impaired growth and a variety of other metabolic disorders in mammals. Only recently has the major role that Se-containing proteins, designated selenoproteins, play in many aspects of health and development begun to emerge. Se is incorporated into protein by way of the Se-containing amino acid, selenocysteine (Sec). The synthesis of selenoproteins is dependent on Sec tRNA for insertion of Sec, the 21st amino acid in the genetic code, into protein. We have taken advantage of this dependency to modulate the expression of Sec tRNA that in turn modulates the expression of selenoproteins by generating transgenic, conditional knockout, transgenic/standard knockout and transgenic/conditional knockout mouse models, all of which involve the Sec tRNA gene, to elucidate the intracellular roles of this protein class.
Keywords: mouse models; selenium; selenocysteine tRNA; selenoproteins

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MDPI and ACS Style

Carlson, B.A.; Yoo, M.-H.; Tsuji, P.A.; Gladyshev, V.N.; Hatfield, D.L. Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development. Molecules 2009, 14, 3509-3527.

AMA Style

Carlson BA, Yoo M-H, Tsuji PA, Gladyshev VN, Hatfield DL. Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development. Molecules. 2009; 14(9):3509-3527.

Chicago/Turabian Style

Carlson, Bradley A.; Yoo, Min-Hyuk; Tsuji, Petra A.; Gladyshev, Vadim N.; Hatfield, Dolph L. 2009. "Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development." Molecules 14, no. 9: 3509-3527.

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