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Synthesis and Theoretical Study of Molecularly Imprinted Nanospheres for Recognition of Tocopherols
Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand
* Authors to whom correspondence should be addressed.
Received: 13 June 2009; in revised form: 5 August 2009 / Accepted: 11 August 2009 / Published: 12 August 2009
Abstract: Molecular imprinting is a technology that facilitates the production of artificial receptors toward compounds of interest. The molecularly imprinted polymers act as artificial antibodies, artificial receptors, or artificial enzymes with the added benefit over their biological counterparts of being highly durable. In this study, we prepared molecularly imprinted polymers for the purpose of binding specifically to tocopherol (vitamin E) and its derivative, tocopherol acetate. Binding of the imprinted polymers to the template was found to be two times greater than that of the control, non-imprinted polymers, when using only 10 mg of polymers. Optimization of the rebinding solvent indicated that ethanol-water at a molar ratio of 6:4 (v/v) was the best solvent system as it enhanced the rebinding performance of the imprinted polymers toward both tocopherol and tocopherol acetate with a binding capacity of approximately 2 mg/g of polymer. Furthermore, imprinted nanospheres against tocopherol was successfully prepared by precipitation polymerization with ethanol-water at a molar ratio of 8:2 (v/v) as the optimal rebinding solvent. Computer simulation was also performed to provide mechanistic insights on the binding mode of template-monomer complexes. Such polymers show high potential for industrial and medical applications, particularly for selective separation of tocopherol and derivatives.
Keywords: tocopherol; vitamin E; molecular imprinting; molecularly imprinted polymer; MIP; molecular modeling
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Piacham, T.; Nantasenamat, C.; Suksrichavalit, T.; Puttipanyalears, C.; Pissawong, T.; Maneewas, S.; Isarankura-Na-Ayudhya, C.; Prachayasittikul, V. Synthesis and Theoretical Study of Molecularly Imprinted Nanospheres for Recognition of Tocopherols. Molecules 2009, 14, 2985-3002.
Piacham T, Nantasenamat C, Suksrichavalit T, Puttipanyalears C, Pissawong T, Maneewas S, Isarankura-Na-Ayudhya C, Prachayasittikul V. Synthesis and Theoretical Study of Molecularly Imprinted Nanospheres for Recognition of Tocopherols. Molecules. 2009; 14(8):2985-3002.
Piacham, Theeraphon; Nantasenamat, Chanin; Suksrichavalit, Thummaruk; Puttipanyalears, Charoenchai; Pissawong, Tippawan; Maneewas, Supanee; Isarankura-Na-Ayudhya, Chartchalerm; Prachayasittikul, Virapong. 2009. "Synthesis and Theoretical Study of Molecularly Imprinted Nanospheres for Recognition of Tocopherols." Molecules 14, no. 8: 2985-3002.