A sensitive method coupling high-performance liquid chromatography (HPLC) with diode-array detector (DAD) and electrospray ionization mass spectrometry (MS) was optimized for the separation and identification of phenolic acids, flavonoid glycosides and flavonoid aglycones in the extract of burr parsley (
Caucalis platycarpos L.). Fragmentation behavior of flavonoid glycosides and phenolic acids were investigated using ion trap mass spectrometry in negative electrospray ionization. The MS, MS
n and UV data together with HPLC retention time (
TR) of phenolic acids and flavonoids allowed structural characterization of these compounds. Caffeoylquinic acid (CQA) isomers,
p-coumaroyl-quinic acids (
p-CoQA), feruloylquinic acids (FQA), dicaffeoylquinic acids (diCQA), luteolin-7-
O-rutinoside, apigenin-7-
O-rutinoside as well as isolated chrysoeriol-7-
O-rutinoside have been identified as constituents of
C. platycarpos for the first time. An accurate, precise and sensitive LC-DAD method for quantification of four phenolic acids (3-
O-caffeoylquinic, caffeic,
p-coumaric,
o-coumaric acid), four flavonoid glycosides (luteolin-7-
O-glucoside, apigenin-7-
O-glucoside, quercetin-3-
O-galactoside, quercetin-3-
O-rhamnoside), and three flavonoid aglycones (luteolin, apigenin, chrysoeriol) in
C. platycarpos extract was validated in terms of linearity, limit of detection, limit of quantification, precision and accuracy. 3-
O-caffeoylquinic acid was the predominant phenolic acid and luteolin-7-
O-glucoside was the predominant flavonoid glycoside.
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