Molecules 2009, 14(7), 2669-2683; doi:10.3390/molecules14072669
Article

Mechanism of Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as Non-Viral Gene Carrier

Yuki Eshita1,* email, Junko Higashihara1 , Masayasu Onishi2 , Masaaki Mizuno3 email, Jun Yoshida4 email, Tomohiko Takasaki5 email, Naoji Kubota6 email and Yasuhiko Onishi2,* email
1 Department of Infectious Disease Control, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu-shi, Oita Prefecture 879-5593, Japan 2 Ryujyu Science Corporation, 39-4 Kosora-cho, Seto-shi, Aichi Prefecture 489-0842, Japan 3 The Center for Genetic and Regenerative Medicine, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya-shi, Aichi Prefecture 466-8550, Japan 4 Department of Neurosurgery, Nagoya University Graduate School of Medicine (65 Tsuruma-cho, Showa-ku, Nagoya-shi, Aichi Prefecture 466-8550, Japan 5 Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjyuku-ku, Tokyo 162-8640, Japan 6 Department of Chemistry, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu-shi, Oita Prefecture 879-5593, Japan
* Authors to whom correspondence should be addressed.
Received: 30 June 2009; in revised form: 8 July 2009 / Accepted: 15 July 2009 / Published: 23 July 2009
(This article belongs to the Special Issue Macromolecules: Chemistry, Medicinal and Functional Materials)
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Abstract: Comparative investigations were carried out regarding the efficiency of introduction of exogenous genes into cultured cells using a cationic polysaccharide DEAE-dextran-MMA (methyl methacrylate ester) graft copolymer (2-diethylaminoethyl-dextran-methyl methacrylate graft copolymer; DDMC) as a nonviral carrier for gene introduction. The results confirmed that the gene introduction efficiency was improved with DDMC relative to DEAE-dextran. Comparative investigations were carried out using various concentrations of DDMC and DNA in the introduction of DNA encoding luciferase (pGL3 control vector; Promega) into COS-7 cells derived from African green monkey kidney cells. The complex formation reaction is thought to be directly proportional to the transformation rate, but the complex formation reaction between DDMC and DNA is significantly influenced by hydrophobic bonding strength along with hydrogen bonding strength and Coulomb forces due to the hydrophobicity of the grafted MMA sections. It is thought that the reaction is a Michaelis-Menten type complex formation reaction described by the following equation: Complex amount = K1 (DNA concentration)(DDMC concentration). In support of this equation, it was confirmed that the amount of formed complex was proportional to the RLU value.
Keywords: transfection efficiency; DEAE-dextran-MMA graft copolymer; non-viral gene carrier; exogenous genes

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Cite This Article

MDPI and ACS Style

Eshita, Y.; Higashihara, J.; Onishi, M.; Mizuno, M.; Yoshida, J.; Takasaki, T.; Kubota, N.; Onishi, Y. Mechanism of Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as Non-Viral Gene Carrier. Molecules 2009, 14, 2669-2683.

AMA Style

Eshita Y., Higashihara J., Onishi M., Mizuno M., Yoshida J., Takasaki T., Kubota N., Onishi Y. Mechanism of Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as Non-Viral Gene Carrier. Molecules. 2009; 14(7):2669-2683.

Chicago/Turabian Style

Eshita, Yuki; Higashihara, Junko; Onishi, Masayasu; Mizuno, Masaaki; Yoshida, Jun; Takasaki, Tomohiko; Kubota, Naoji; Onishi, Yasuhiko. 2009. "Mechanism of Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as Non-Viral Gene Carrier." Molecules 14, no. 7: 2669-2683.

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