Molecules 2009, 14(6), 2043-2048; doi:10.3390/molecules14062043
Communication

Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase

Chang-Chun Ruan 1,2,† email, Hao Zhang 3,† email, Lian-Xue Zhang 1, Zhi Liu 2 email, Guang-Zhi Sun 2 email, Jun Lei 1 email, Yu-Xia Qin 4 email, Yi-Nan Zheng 1 email, Xiang Li 5,* email and Hong-Yu Pan 6,* email
1 College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China 2 Institute of Agricultural Modernization, Jilin Agricultural University, Changchun, 130118, China 3 School of Resources and Environment, Jilin Agricultural University, Changchun, 130118, China 4 Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China 5 Saskatoon Research Center, Agriculture and Agri-food Canada, Saskatoon, SK S7N 0X2, Canada 6 College of Plant Science, Jilin University, Changchun, 130062, China † These authors contributed equally and are considered as co-first authors
* Authors to whom correspondence should be addressed.
Received: 6 March 2009; in revised form: 10 May 2009 / Accepted: 31 May 2009 / Published: 8 June 2009
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Abstract: An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (~170 kD) showed the ability to transform ginsenoside Rf into Rh1.
Keywords: Aspergillus niger; β-glucosidase; ginsenoside Rf; ginsenoside Rh1

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Cite This Article

MDPI and ACS Style

Ruan, C.-C.; Zhang, H.; Zhang, L.-X.; Liu, Z.; Sun, G.-Z.; Lei, J.; Qin, Y.-X.; Zheng, Y.-N.; Li, X.; Pan, H.-Y. Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase. Molecules 2009, 14, 2043-2048.

AMA Style

Ruan C.-C., Zhang H., Zhang L.-X., Liu Z., Sun G.-Z., Lei J., Qin Y.-X., Zheng Y.-N., Li X., Pan H.-Y. Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase. Molecules. 2009; 14(6):2043-2048.

Chicago/Turabian Style

Ruan, Chang-Chun; Zhang, Hao; Zhang, Lian-Xue; Liu, Zhi; Sun, Guang-Zhi; Lei, Jun; Qin, Yu-Xia; Zheng, Yi-Nan; Li, Xiang; Pan, Hong-Yu. 2009. "Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase." Molecules 14, no. 6: 2043-2048.

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