Molecules 2009, 14(4), 1614-1626; doi:10.3390/molecules14041614
Article

Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins

Dana Stan 1,* email, Iulia Matei 2 email, Carmen Mihailescu 1 email, Mihaela Savin 3 email, Mihaela Matache 1 email, Mihaela Hillebrand 2 email and Ion Baciu 1 email
1 Department of Organic Chemistry, Bucharest University, 00133, Sos. Panduri No. 90-92, Romania 2 Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, No. 4-12, Bucharest, Romania 3 Telemedica, Str. Ion Calin, No.13, Bucharest, Romania
* Author to whom correspondence should be addressed.
Received: 17 March 2009; in revised form: 15 April 2009 / Accepted: 20 April 2009 / Published: 24 April 2009
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Abstract: Binding of a newly synthesized indanedione derivative, 2-(2-hydroxy-3-ethoxybenzylidene)-1,3-indanedione (HEBID), to human and bovine serum albumins (HSA and BSA), under simulated physiological conditions was monitored by fluorescence spectroscopy. The binding parameters (binding constants and number of binding sites) and quenching constants were determined according to literature models. The quenching mechanism was assigned to a Förster non-radiative energy transfer due to the HEBID-SA complex formation. A slightly increased affinity of HEBID for HSA was found, while the number of binding sites is approximately one for both albumins. The molecular distance between donor (albumin) and acceptor (HEBID) and the energy transfer efficiency were estimated, in the view of Förster’s theory. The effect of HEBID on the protein conformation was investigated using circular dichroism and synchronous fluorescence spectroscopies. The results revealed partial unfolding in the albumins upon interaction, as well as changes in the local polarity around the tryptophan residues
Keywords: Indanedione; Serum albumin; Fluorescence; Circular dichroism; Fluorescence resonance energy transfer

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MDPI and ACS Style

Stan, D.; Matei, I.; Mihailescu, C.; Savin, M.; Matache, M.; Hillebrand, M.; Baciu, I. Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins. Molecules 2009, 14, 1614-1626.

AMA Style

Stan D, Matei I, Mihailescu C, Savin M, Matache M, Hillebrand M, Baciu I. Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins. Molecules. 2009; 14(4):1614-1626.

Chicago/Turabian Style

Stan, Dana; Matei, Iulia; Mihailescu, Carmen; Savin, Mihaela; Matache, Mihaela; Hillebrand, Mihaela; Baciu, Ion. 2009. "Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins." Molecules 14, no. 4: 1614-1626.

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