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Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins
Department of Organic Chemistry, Bucharest University, 00133, Sos. Panduri No. 90-92, Romania
Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, No. 4-12, Bucharest, Romania
Telemedica, Str. Ion Calin, No.13, Bucharest, Romania
* Author to whom correspondence should be addressed.
Received: 17 March 2009; in revised form: 15 April 2009 / Accepted: 20 April 2009 / Published: 24 April 2009
Abstract: Binding of a newly synthesized indanedione derivative, 2-(2-hydroxy-3-ethoxybenzylidene)-1,3-indanedione (HEBID), to human and bovine serum albumins (HSA and BSA), under simulated physiological conditions was monitored by fluorescence spectroscopy. The binding parameters (binding constants and number of binding sites) and quenching constants were determined according to literature models. The quenching mechanism was assigned to a Förster non-radiative energy transfer due to the HEBID-SA complex formation. A slightly increased affinity of HEBID for HSA was found, while the number of binding sites is approximately one for both albumins. The molecular distance between donor (albumin) and acceptor (HEBID) and the energy transfer efficiency were estimated, in the view of Förster’s theory. The effect of HEBID on the protein conformation was investigated using circular dichroism and synchronous fluorescence spectroscopies. The results revealed partial unfolding in the albumins upon interaction, as well as changes in the local polarity around the tryptophan residues
Keywords: Indanedione; Serum albumin; Fluorescence; Circular dichroism; Fluorescence resonance energy transfer
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Stan, D.; Matei, I.; Mihailescu, C.; Savin, M.; Matache, M.; Hillebrand, M.; Baciu, I. Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins. Molecules 2009, 14, 1614-1626.
Stan D, Matei I, Mihailescu C, Savin M, Matache M, Hillebrand M, Baciu I. Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins. Molecules. 2009; 14(4):1614-1626.
Stan, Dana; Matei, Iulia; Mihailescu, Carmen; Savin, Mihaela; Matache, Mihaela; Hillebrand, Mihaela; Baciu, Ion. 2009. "Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins." Molecules 14, no. 4: 1614-1626.