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The Shorter the Better: Reducing Fixed Primer Regions of Oligonucleotide Libraries for Aptamer Selection
Departments of Pathology, Biochemistry & Molecular Biology, College of Medicine, Pennsylvania State University, Hershey, PA 17033, USA
Gittlen Cancer Research Foundation, Hershey Medical Center, Pennsylvania State University, 500 University Drive, Hershey, PA 17033, USA
* Author to whom correspondence should be addressed.
Received: 20 February 2009; in revised form: 18 March 2009 / Accepted: 25 March 2009 / Published: 27 March 2009
Abstract: Oligonucleotide aptamers are highly structured DNA or RNA molecules, or modified versions thereof, that can bind to targets with specific affinities comparable to antibodies. They are identified through an in vitro selection process termed SELEX (Systematic Evolution of Ligands by EXponential enrichment) to recognize a wide variety of targets, from small molecules to proteins, and from cultured cells to whole organisms. Aptamers possess a number of desirable properties, such as ease of synthesis, stability, robustness, and lack of immunogenicity. Standard SELEX libraries require two primers, one on each side of a central random domain, to amplify the target-bound sequences via PCR or RT-PCR. However, these primer sequences cause non-specific binding by their nature, and have been reported to lead to large numbers of false-positive binding sequences, or to interfere with binding of sequences within the random regions. This review is focused on methods which have been developed to eliminate fixed primer interference during the SELEX process.
Keywords: Aptamer; SELEX
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MDPI and ACS Style
Pan, W.; Clawson, G.A. The Shorter the Better: Reducing Fixed Primer Regions of Oligonucleotide Libraries for Aptamer Selection. Molecules 2009, 14, 1353-1369.
Pan W, Clawson GA. The Shorter the Better: Reducing Fixed Primer Regions of Oligonucleotide Libraries for Aptamer Selection. Molecules. 2009; 14(4):1353-1369.
Pan, Weihua; Clawson, Gary A. 2009. "The Shorter the Better: Reducing Fixed Primer Regions of Oligonucleotide Libraries for Aptamer Selection." Molecules 14, no. 4: 1353-1369.