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Molecules 2009, 14(3), 959-969; doi:10.3390/molecules14030959
Article

Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells

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Received: 4 December 2008; in revised form: 7 February 2009 / Accepted: 17 February 2009 / Published: 2 March 2009
(This article belongs to the Special Issue Phenolics and Polyphenolics)
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Abstract: The effect of [10]-gingerol on cytosol free Ca2+ concentration ([Ca2+]i) and viability is large unknown. This study examines the early signaling effects of [10]-gingerol on human colorectal cancer cells. It was found that this compound caused a slow and sustained rise of [Ca2+]i in a concentration-dependent manner. [10]-Gingerol also induced a [Ca2+]i rise when extracellular Ca2+ was removed, but the magnitude was reduced by 38%. In a Ca2+-free medium, the [10]-gingerol-induced [Ca2+]i rise was partially abolished by depleting stored Ca2+ with thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor). The elevation of [10]-gingerol-caused [Ca2+]i in a Ca2+-containing medium was not affected by modulation of protein kinase C activity. The [10]-gingerol-induced Ca2+ influx was insensitive to L-type Ca2+ channel blockers. At concentrations of 10-100 mM, [10]-gingerol killed cells in a concentration-dependent manner. These findings suggest that [10]-gingerol induces [Ca2+]i rise by causing Ca2+ release from the endoplasmic reticulum and Ca2+ influx from non-L-type Ca2+ channels in SW480 cancer cells.
Keywords: Ca2+; [10]-Gingerol; L-type Ca2+ channel blockers; SW480 cells; Thapsigargin Ca2+; [10]-Gingerol; L-type Ca2+ channel blockers; SW480 cells; Thapsigargin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chen, C.-Y.; Li, Y.-W.; Kuo, S.-Y. Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells. Molecules 2009, 14, 959-969.

AMA Style

Chen C-Y, Li Y-W, Kuo S-Y. Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells. Molecules. 2009; 14(3):959-969.

Chicago/Turabian Style

Chen, Chung-Yi; Li, Yi-Wen; Kuo, Soong-Yu. 2009. "Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells." Molecules 14, no. 3: 959-969.


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