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Effect of -Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells
Department of Medical Technology, School of Medicine and Health Sciences, Fooyin University, Kaohsiung County 83101 Taiwan
* Author to whom correspondence should be addressed.
Received: 4 December 2008; in revised form: 7 February 2009 / Accepted: 17 February 2009 / Published: 2 March 2009
Abstract: The effect of -gingerol on cytosol free Ca2+ concentration ([Ca2+]i) and viability is large unknown. This study examines the early signaling effects of -gingerol on human colorectal cancer cells. It was found that this compound caused a slow and sustained rise of [Ca2+]i in a concentration-dependent manner. -Gingerol also induced a [Ca2+]i rise when extracellular Ca2+ was removed, but the magnitude was reduced by 38%. In a Ca2+-free medium, the -gingerol-induced [Ca2+]i rise was partially abolished by depleting stored Ca2+ with thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor). The elevation of -gingerol-caused [Ca2+]i in a Ca2+-containing medium was not affected by modulation of protein kinase C activity. The -gingerol-induced Ca2+ influx was insensitive to L-type Ca2+ channel blockers. At concentrations of 10-100 mM, -gingerol killed cells in a concentration-dependent manner. These findings suggest that -gingerol induces [Ca2+]i rise by causing Ca2+ release from the endoplasmic reticulum and Ca2+ influx from non-L-type Ca2+ channels in SW480 cancer cells.
Keywords: Ca2+; -Gingerol; L-type Ca2+ channel blockers; SW480 cells; Thapsigargin
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MDPI and ACS Style
Chen, C.-Y.; Li, Y.-W.; Kuo, S.-Y. Effect of -Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells. Molecules 2009, 14, 959-969.
Chen C-Y, Li Y-W, Kuo S-Y. Effect of -Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells. Molecules. 2009; 14(3):959-969.
Chen, Chung-Yi; Li, Yi-Wen; Kuo, Soong-Yu. 2009. "Effect of -Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells." Molecules 14, no. 3: 959-969.