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High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity
UNESP-Univ Estadual Paulista, Instituto de Química, Departamento de Química Orgânica, CP 355, CEP 14800-900, Araraquara, São Paulo, Brazil
UEMS, Course of Chemistry, Mato Grosso do Sul State University, CP 351, CEP 79804-970, Dourados, Mato Grosso do Sul, Brazil
* Author to whom correspondence should be addressed.
Received: 9 October 2009; in revised form: 13 November 2009 / Accepted: 16 November 2009 / Published: 16 November 2009
Abstract: Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-β-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines.
Keywords: eriocaulaceae; liquid chromatography; quantification; flavonoids
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Da Silva, M.A.; Cardoso, C.A.L.; Vilegas, W.; Dos Santos, L.C. High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity. Molecules 2009, 14, 4644-4654.
Da Silva MA, Cardoso CAL, Vilegas W, Dos Santos LC. High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity. Molecules. 2009; 14(11):4644-4654.
Da Silva, Marcelo A.; Cardoso, Claudia Andréa L.; Vilegas, Wagner; Dos Santos, Lourdes C. 2009. "High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity." Molecules 14, no. 11: 4644-4654.