Next Article in Journal
A Selective Pharmacophore Model for β2-Adrenoceptor Agonists
Previous Article in Journal
13C-NMR Data of Three Important Diterpenes Isolated from Euphorbia Species
Molecules 2009, 14(11), 4476-4485; doi:10.3390/molecules14114476
Article

In Vitro Antioxidant and Xanthine Oxidase Inhibitory Activities of Methanolic Swietenia mahagoni Seed Extracts

1, 1, 2,* , 1, 1 and 1
Received: 4 September 2009; in revised form: 10 October 2009 / Accepted: 27 October 2009 / Published: 6 November 2009
Download PDF [272 KB, uploaded 18 June 2014]
Abstract: This study examines the in vitro antioxidant activities of the methanol extract of Swietenia mahagoni seeds (SMCM seed extract). The extract was screened for possible antioxidant activities by free radical scavenging activity (DPPH), xanthine oxidase inhibition (XOI), hydrogen peroxide scavenging activity (HPSA) and ferric-reducing antioxidant power (FRAP) assays. The total phenolic and flavonoid contents were also determined. The extract exhibits antioxidant activity of 23.29% with an IC50 value of 2.3 mg/mL in the DPPH radical scavenging method, 47.2% in the XOI assay, 49.5% by the HPSA method, and 0.728 mmol/Fe(II)g in the FRAP method at the concentration tested. The amount of total phenolics and flavonoid contents was 70.83 mg gallic acid equivalent (GAE) and 2.5 ± 0.15 mg of catechin equivalent per gram of dry extract, respectively. High Performance Thin Layer Chromatography (HPTLC) screening indicates the presence of phenolic compounds in the SMCM seed extract. The results indicate that the extract has both high free radical scavenging and xanthine oxidase inhibition activity. The antioxidant activity of SMCM seed extract is comparable with that of other Malaysian tropical fruits and herbal plants.
Keywords: Swietenia mahagoni seed; antioxidant activity; HPTLC Swietenia mahagoni seed; antioxidant activity; HPTLC
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Sahgal, G.; Ramanathan, S.; Sasidharan, S.; Mordi, M.N.; Ismail, S.; Mansor, S.M. In Vitro Antioxidant and Xanthine Oxidase Inhibitory Activities of Methanolic Swietenia mahagoni Seed Extracts. Molecules 2009, 14, 4476-4485.

AMA Style

Sahgal G, Ramanathan S, Sasidharan S, Mordi MN, Ismail S, Mansor SM. In Vitro Antioxidant and Xanthine Oxidase Inhibitory Activities of Methanolic Swietenia mahagoni Seed Extracts. Molecules. 2009; 14(11):4476-4485.

Chicago/Turabian Style

Sahgal, Geethaa; Ramanathan, Surash; Sasidharan, Sreenivasan; Mordi, Mohd Nizam; Ismail, Sabariah; Mansor, Sharif Mahsufi. 2009. "In Vitro Antioxidant and Xanthine Oxidase Inhibitory Activities of Methanolic Swietenia mahagoni Seed Extracts." Molecules 14, no. 11: 4476-4485.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert