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Molecules 2009, 14(10), 3942-3951; doi:10.3390/molecules14103942

Separation, Characterization and Dose-Effect Relationship of the PPARγ-Activating Bio-Active Constituents in the Chinese Herb Formulation ‘San-Ao Decoction’

1
Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China
2
Nanjing Medical University, Nanjing 210029, China
*
Authors to whom correspondence should be addressed.
Received: 14 August 2009 / Revised: 9 September 2009 / Accepted: 29 September 2009 / Published: 9 October 2009
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Abstract

San-ao decoction (SAD), comprising Herba Ephedrae, Radix et Rhizoma Glycyrrhizae and Seneb Armeniacae Amarum, is one of the most popular traditional Chinese medicine (TCM) formulae for asthma. Peroxisome proliferator-activated receptors (PPARs) areey regulators of lipid and glucose metabolism and have become important therapeutic targets for various deseases, PPARγ activation might exhibit anti-inflammatory properties in different chronic inflammatory processes. The EtOAc fraction of SAD showed a significant effect on PPARγ activation. A simple and rapid method has been established for separation and characterization of the main compounds in the PPARγ-activating fraction of SAD by ultra-fast HPLC coupled with quadropole time-of-flight mass pectrometry (UPLC-Q-TOF/MS). A total of 10 compounds were identified in the activating fraction of SAD, including amygdalin (1), liquiritin (2), 6′-acetyliquiritin (3), liquiritigenin (4), isoliquiritigenin (5), formononetin (6), licoisoflavanone (7), glycycoumarin (8), glycyrol (9) and uercetin (10). The results also characterized formononetin as a predominant component in this fraction. The dose-effect relationship comparison study of formononetin and the EtOAc fraction of SAD by adding formononetin was performed, the results suggested that formononetin was the major component of the EtOAc fraction of SAD responsible for activating PPARγ, and the method will possibly be applied to study the complex biological active constituents of other TCMs.
Keywords: San-ao decoction; PPARγ; does-effect; UPLC; quatropde time-of-flight mass spectrometry San-ao decoction; PPARγ; does-effect; UPLC; quatropde time-of-flight mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zhou, L.; Tang, Y.-P.; Gao, L.; Fan, X.-S.; Liu, C.-M.; Wu, D.-K. Separation, Characterization and Dose-Effect Relationship of the PPARγ-Activating Bio-Active Constituents in the Chinese Herb Formulation ‘San-Ao Decoction’. Molecules 2009, 14, 3942-3951.

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