Molecules 2008, 13(8), 1538-1550; doi:10.3390/molecules13081538
Article

Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry

Wei-Ting Hung 1 , Shwu-Huey Wang 2 , Chung-Hsuan Chen 1  and Wen-Bin Yang 1,* email
1 Genomics Research Center, Academia Sinica, No. 128, Academia Road Section 2, Nan-Kang, Taipei 11529, Taiwan 2 Instrument Center, Taipei Medical University, Taipei 11031, Taiwan
* Author to whom correspondence should be addressed.
Received: 26 May 2008; in revised form: 21 June 2008 / Accepted: 25 July 2008 / Published: 3 August 2008
(This article belongs to the Special Issue Polysaccharides: Organic Chemistry, Bioactivity and Analysis, including Those from Medicinal Plants and Fungi)
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Abstract: A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, β-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lucidum, dextran, curdlan and maltohexaose were also pursued and different fragment patterns were obtained. The G. lucidum glucan structure was determined and this method for linkage analysis of permethylated glucan has been proven feasible.
Keywords: Polysaccharides; matrix-assisted laser desorption/ionization (MALDI); Ganoderma lucidum; β-glucan; 2; 5-dihydroxybenzoic acid (2; 5-DHB).

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MDPI and ACS Style

Hung, W.-T.; Wang, S.-H.; Chen, C.-H.; Yang, W.-B. Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry. Molecules 2008, 13, 1538-1550.

AMA Style

Hung W-T, Wang S-H, Chen C-H, Yang W-B. Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry. Molecules. 2008; 13(8):1538-1550.

Chicago/Turabian Style

Hung, Wei-Ting; Wang, Shwu-Huey; Chen, Chung-Hsuan; Yang, Wen-Bin. 2008. "Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry." Molecules 13, no. 8: 1538-1550.

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