Molecules 2008, 13(8), 1538-1550; doi:10.3390/molecules13081538
Article

Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry

1, 2, 1 and 1,* email
Received: 26 May 2008; in revised form: 21 June 2008 / Accepted: 25 July 2008 / Published: 3 August 2008
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, β-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lucidum, dextran, curdlan and maltohexaose were also pursued and different fragment patterns were obtained. The G. lucidum glucan structure was determined and this method for linkage analysis of permethylated glucan has been proven feasible.
Keywords: Polysaccharides; matrix-assisted laser desorption/ionization (MALDI); Ganoderma lucidum; β-glucan; 2; 5-dihydroxybenzoic acid (2; 5-DHB).
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MDPI and ACS Style

Hung, W.-T.; Wang, S.-H.; Chen, C.-H.; Yang, W.-B. Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry. Molecules 2008, 13, 1538-1550.

AMA Style

Hung W-T, Wang S-H, Chen C-H, Yang W-B. Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry. Molecules. 2008; 13(8):1538-1550.

Chicago/Turabian Style

Hung, Wei-Ting; Wang, Shwu-Huey; Chen, Chung-Hsuan; Yang, Wen-Bin. 2008. "Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry." Molecules 13, no. 8: 1538-1550.

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