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Molecules 2008, 13(8), 1538-1550; doi:10.3390/molecules13081538

Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry

1
Genomics Research Center, Academia Sinica, No. 128, Academia Road Section 2, Nan-Kang, Taipei 11529, Taiwan
2
Instrument Center, Taipei Medical University, Taipei 11031, Taiwan
*
Author to whom correspondence should be addressed.
Received: 26 May 2008 / Revised: 21 June 2008 / Accepted: 25 July 2008 / Published: 3 August 2008
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Abstract

A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, β-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lucidum, dextran, curdlan and maltohexaose were also pursued and different fragment patterns were obtained. The G. lucidum glucan structure was determined and this method for linkage analysis of permethylated glucan has been proven feasible. View Full-Text
Keywords: Polysaccharides; matrix-assisted laser desorption/ionization (MALDI); Ganoderma lucidum; β-glucan; 2; 5-dihydroxybenzoic acid (2; 5-DHB). Polysaccharides; matrix-assisted laser desorption/ionization (MALDI); Ganoderma lucidum; β-glucan; 2; 5-dihydroxybenzoic acid (2; 5-DHB).
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Hung, W.-T.; Wang, S.-H.; Chen, C.-H.; Yang, W.-B. Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry. Molecules 2008, 13, 1538-1550.

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