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Phenolic Compounds in Field Horsetail (Equisetum arvense L.) as Natural Antioxidants
Department of Chemistry, Faculty of Sciences, Trg D. Obradovica 3, University of Novi Sad, 21000 Novi Sad, Serbia
Department of Pharmacy, Faculty of Medicine, Hajduk Veljkova 3, University of Novi Sad, 21000 Novi Sad, Serbia
* Author to whom correspondence should be addressed.
Received: 12 June 2008; in revised form: 1 July 2008 / Accepted: 15 July 2008 / Published: 17 July 2008
Abstract: In this paper, the study of antioxidant activity and phenolic composition of three different extracts (EtOAc, n-BuOH and H2O) of field horsetail (Equisetum arvense L.) is presented. The antioxidant activity has been evaluated measuring the total reducing power (expressed by Ascorbate Equivalent Antioxidant Capacity - AEAC), inhibition of lipid peroxidation, and free radical scavenging capacity (RSC) towards 2,2-diphenyl-1- picrylhydrazyl (DPPH radical) and nitric oxide (NO), respectively. In addition, the total flavonoid content (TFC) and phenolic constituents of each extract have been determined. The results obtained show that the highest RSC regarding both DPPH and NO radicals is expressed by EtOAc extract (EC50=2.37 μg/mL and EC50=90.07 μg/mL, respectively), and the lowest by H2O extract (EC50=37.2 μg/mL and EC50>333.33 μg/mL, respectively). n- BuOH extract showed the highest total reducing power (AEAC=13.40 μg/mL). Differences in the phenolic composition of examined extracts are found comparing the HPLC chemical profiles. Although, isoquercitrin is the main flavonoid in both EtOAc and n-BuOH extracts, a considerable amount of di-E-caffeoyl-meso-tartaric acid was presented in the n-BuOH extract. In H2O extract high content of phenolic acids and low percentage of flavonoids were detected.
Keywords: Equisetum arvense L.; NO; DPPH; LP; reducing power
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Mimica-Dukic, N.; Simin, N.; Cvejic, J.; Jovin, E.; Orcic, D.; Bozin, B. Phenolic Compounds in Field Horsetail (Equisetum arvense L.) as Natural Antioxidants. Molecules 2008, 13, 1455-1464.
Mimica-Dukic N, Simin N, Cvejic J, Jovin E, Orcic D, Bozin B. Phenolic Compounds in Field Horsetail (Equisetum arvense L.) as Natural Antioxidants. Molecules. 2008; 13(7):1455-1464.
Mimica-Dukic, Neda; Simin, Natasa; Cvejic, Jelena; Jovin, Emilija; Orcic, Dejan; Bozin, Biljana. 2008. "Phenolic Compounds in Field Horsetail (Equisetum arvense L.) as Natural Antioxidants." Molecules 13, no. 7: 1455-1464.