Next Article in Journal
Effects of Two Terpene Alcohols on the Antibacterial Activity and the Mode of Action of Farnesol against Staphylococcus aureus
Previous Article in Journal
Copper Complexes of Nicotinic-Aromatic Carboxylic Acids as Superoxide Dismutase Mimetics
Molecules 2008, 13(12), 3057-3068; doi:10.3390/molecules13123057

An Emulsion System Based on a Chip Polymerase Chain Reaction

1 Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing, 210096, P.R. China 2 State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, P.R. China
* Author to whom correspondence should be addressed.
Received: 12 November 2008 / Revised: 30 November 2008 / Accepted: 2 December 2008 / Published: 9 December 2008
View Full-Text   |   Download PDF [748 KB, uploaded 18 June 2014]   |  


In this paper we describe a novel method for detecting many DNA fragments through efficient amplification by using an emulsion system based on “on-chip” PCR instead of conventional multiplex polymerase chain reaction (PCR). During the preparation of on-chip PCR, a set of primers were immobilized on a slide and other sets were in an emulsion system. Different emulsion phase primers and other related PCR components were dispersed in different droplets of the emulsion system, and then, due to the thermal instability of emulsion droplets, they would be released onto the surface of the slide after preheating in the first PCR step. To test the above method, we used plasma DNAs from pregnant women who was carrying a male fetus for gender identification. Four different Y chromosome DNA fragments were selected. Results showed that different DNA fragments could be simultaneously amplified with satisfactory results. It is suggested that a simple, convenient and inexpensive on-chip PCR method has been developed.
Keywords: PCR amplification; Emulsion; Immobilized primers PCR amplification; Emulsion; Immobilized primers
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Ge, Q.; Yu, P.; Bai, Y.; Lu, Z. An Emulsion System Based on a Chip Polymerase Chain Reaction. Molecules 2008, 13, 3057-3068.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert