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Molecules 2008, 13(12), 2986-2997; doi:10.3390/molecules13122986

HPLC, NMR and MALDI-TOF MS Analysis of Condensed Tannins from Lithocarpus glaber Leaves with Potent Free Radical Scavenging Activity

1
Key Lab of Ministry of Education for Coast and Wetland Ecosystems, Xiamen 361005, P.R. China
2
Department of Biology, School of Life Sciences, Xiamen University, Xiamen 361005, P.R. China
*
Author to whom correspondence should be addressed.
Received: 5 September 2008 / Revised: 23 November 2008 / Accepted: 2 December 2008 / Published: 4 December 2008
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Abstract

Using acid-catalyzed degradation in the presence of cysteamine, the condensed tannins from Lithocarpus glaber leaves were characterized, following thiolysis, by means of reversed-phase HPLC, 13C-NMR and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) analyses. The thiolysis reaction products showed the presence of the procyanidin (PC) and prodelphinidin (PD) structures. The 13C-NMR spectrum revealed that the condensed tannins were comprised of PD (72.4%) and PC (27.6%), and with a greater content of cis configuration rather than the trans configuration of C2–C3. The MALDI-TOF MS analysis proved the presence of PD units, and the maximum degree of polymerization (DP) was an undecamer. The antioxidant activity of condensed tannins from L. glaber leaves was evaluated by using a free radical scavenging activity assay.
Keywords: Lithocarpus glaber; Condensed tannins; Thiolysis; MALDI-TOF; Free radical scavenging activity Lithocarpus glaber; Condensed tannins; Thiolysis; MALDI-TOF; Free radical scavenging activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zhang, L.L.; Lin, Y.M. HPLC, NMR and MALDI-TOF MS Analysis of Condensed Tannins from Lithocarpus glaber Leaves with Potent Free Radical Scavenging Activity. Molecules 2008, 13, 2986-2997.

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