Next Article in Journal
Substituted N-Phenylpyrazine-2-carboxamides, Their Synthesis and Evaluation as Herbicides and Abiotic Elicitors
Next Article in Special Issue
Trimethyl Lock: A Stable Chromogenic Substrate for Esterases
Previous Article in Journal
Essential Oils of Satureja Species: Insecticidal Effect on Culex pipiens Larvae (Diptera: Culicidae)
Previous Article in Special Issue
The Drug Release Profile from Calcium-induced Alginate Gel Beads Coated with an Alginate Hydrolysate
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Synthesis of 1-Aryl-3-phenethylamino-1-propanone Hydrochlorides as Possible Potent Cytotoxic Agents

1
Department of Chemistry, Faculty of Arts and Sciences, Ataturk University, 25240, Erzurum, Turkey
2
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey
*
Author to whom correspondence should be addressed.
Molecules 2007, 12(12), 2579-2588; https://doi.org/10.3390/12122579
Submission received: 29 October 2007 / Revised: 26 November 2007 / Accepted: 10 December 2007 / Published: 12 December 2007
(This article belongs to the Special Issue Prodrugs)

Abstract

:
1-Aryl-3-phenethylamino-1-propanone hydrochlorides 1-10, which are potential potent cytotoxic agents, were synthesized via Mannich reactions using paraformaldehyde, phenethylamine hydrochloride as the amine component and acetophenone, 4’-methyl-, 4’-methoxy-, 4’-chloro-, 4’-fluoro-, 4’-bromo-, 2’,4’-dichloro-, 4’-nitro-, 4’-hydroxyacetophenone or 2-acetylthiophene as the ketone component. Yields were in the 87-98 % range. Of the compounds synthesized, compounds 2, 6-8 and 10 were new. The optimum reaction conditions were investigated by changing the mol ratios of the reactants, the solvents and the acidity levels using 1 and 10 as representative targets. It was observed that the best mol ratio of the ketone, paraformaldehyde and phenethylamine hydrochloride was 1:1.2:1 (compared with a 2:2.1 ratio), and the most suitable reaction medium was ethanol containing concentrated hydrochloric acid (compared with only ethanol or no solvent). This study may serve as a guide for the conditions of the reactions to synthesize compounds having similar chemical structures.

Introduction

So-called Mannich bases are generally formed by the reaction between formaldehyde, a secondary amine and a compound containing reactive hydrogen atoms. On occasion, aldehydes other than formaldehyde may be employed and the secondary amine may be replaced by ammonia and primary amines. This process is known as the Mannich reaction [1]. Mannich bases display varied biological activities such as antimicrobial, cytotoxic, anticancer, analgesic, anti-inflammatory, diuretic and anticonvulsant properties [2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25]. The deamination process is important to evoke biological responses. Thus, an aminoketone possessing at least one activated hydrogen atom β to the amino group can undergo deamination in vivo or under simulated in vitro conditions to generate the corresponding α,β-unsaturated ketone. The biological activities of Mannich bases have been attributed to these liberated α,β-unsaturated ketones, which can alkylate nucleophiles, especially thiol groups [2,5,9,10,14,26].
The key interests of our laboratory are the design, synthesis and evaluation of the biological activities of Mannich bases generally derived from acetophenones. We have previously reported the synthesis and diverse biological activities of some 3-amino-1-aryl-1-propanone hydrochloride mono-Mannich bases, in which the amine moiety was varied between dimethylamine, piperidine and morpholine, etc. [2,4,6,7,8,9,10,11,12,14,24,25,26]. Since we observed considerable biological activities in these compounds, we now aimed to design and synthesize some new 1-aryl-3-phenethylamino-1-propanone hydrochloride mono-Mannich bases, which are potential prodrugs of potent thiol-alkylating cytotoxic compounds.

Results and Discussion

The synthetic route used for the preparation of the compounds 1-10 designed for this study is shown in Scheme 1.
Scheme 1. Synthetic pathway for the synthesis of the compounds 1-10.
Scheme 1. Synthetic pathway for the synthesis of the compounds 1-10.
Molecules 12 02579 g002
The structures of the compounds thus prepared are shown in Figure 1. Of the compounds synthesized, compounds 2, 6-8, and 10 were new, while compounds 1, 3-5, and 9 have been previously reported [16,27,28,29]. The structures of the synthesized compounds were confirmed by 1H- and 13C-NMR (Table 1), UV, IR and MS data (Table 2). The purities of the compounds were confirmed by elemental (C, H, N) analysis, which were within 0.4 % of the calculated values, with the sole exception of compound 9 (calculated: C: 66.77, H: 6.59, N: 4.58; found: C: 67.08, H: 6.36, N: 3.27).
Figure 1. Chemical structures of the synthesized compounds 1 -10.
Figure 1. Chemical structures of the synthesized compounds 1 -10.
Molecules 12 02579 g001
Ar: 1 = C6H5; 2 = 4'-CH3C6H4; 3 = 4'-CH3OC6H4; 4 = 4'-ClC6H4; 5 = 4'-FC6H4; 6 = 4'-BrC6H4; 7 = 2',4'-(Cl)2C6H3); 8 = 4'-NO2C6H4; 9 = 4'-HOC6H4; 10 = C4H3S(2-yl).
Table 1. 1H- and 13C-NMR spectra of the synthesized compounds 1-10.
Table 1. 1H- and 13C-NMR spectra of the synthesized compounds 1-10.
Compound1H-NMR (DMSO-d6)13 C-NMR (DMSO-d6)
1δ 2.96-3.17 (2H, m), 3.18-3.21 (2H, m), 3.28 (2H, t, J = 6.7 Hz), 3.55 (2H, t, J = 6.9 Hz), 7.22-7.34 (5H, m), 7.53-7.69 (3H, m), 7.96-7.98 (2H, m), 9.23 (1H, br s).δ 32.2, 35.1, 42.4, 48.4, 127.4, 128.6, 129.31, 129.34, 129.5, 134.4, 136.5, 137.9, 197.6.
2δ 2.37 (1H, s), 2.48-2.99 (2H, m), 3.18-3.37 (4H, m), 3.49 (2H, t, J = 6.9 Hz), 7.24-7.36 (7H, m), 7.87 (2H, d, J = 8.0 Hz ), 9.10 (1H, br s).δ 21.8, 32.2, 34.9, 42.5, 48.4, 127.4, 128.7, 129.3, 129.4, 130.0, 134.1, 137.9, 144.8, 197.1.
3δ 2.96-3.00 (2H, m), 3.16-3.20 (2H, m), 3.26 (2H, t, J = 6.9 Hz), 3.47 (2H, t, J = 6.7 Hz), 3.83 (3H, s), 7.94 (2H, d, J = 8.7 Hz ), 7.06 (2H, d, J = 8.7 Hz ), 7.22-7.34 (5H, m), 9.16 (1H, br s).δ 32.2, 34.7, 42.6, 48.4, 56.3, 114.7, 127.4, 129.31, 129.33, 129.5, 131.0, 137.9, 164.2, 195.9.
4δ 2.94-2.99 (2H, m), 3.17-3.21 (2H, m), 3.28 (2H, t, J = 6.5 Hz), 3.52 (2H, t, J = 6.7 Hz), 7.22-7.34 (5H, m), 7.63 (2H, d, J = 8.4 Hz ), 7.98 (2H, d, J = 8.7 Hz ), 9.10 (1H, br s).δ 32.2, 35.1, 42.3, 48.4, 127.4, 129.3, 129.4, 129.6, 130.6, 135.3, 137.9, 139.2, 196.7.
5δ 2.95-2.99 (2H, m), 3.16-3.20 (2H, m), 3.27 (2H, t, J = 6.7 Hz), 3.53 (2H, t, J = 6.9 Hz), 7.21-7.41 (7H, m), 8.05 (2H, dd, J = 8.7, 5.4 Hz ), 9.18 (1H, br s).δ 32.2, 35.0, 42.4, 48.4, 116.5, 127.4, 129.3, 131.7, 133.3, 137.9, 164.7, 167.2, 196.2.
6δ 2.95-3.00 (2H, m), 3.16-3.20 (2H, m), 3.27 (2H, t, J = 6.6 Hz), 3.52 (2H, t, J = 6.8 Hz), 7.22-7.34 (5H, m), 7.76 (2H, d, J = 8.4 Hz ), 7.90 (2H, d, J = 8.4 Hz ), 9.20 (1H, br s).δ 32.2, 35.1, 42.3, 48.4, 127.4, 128.4, 129.31, 129.34, 130.6, 132.6, 135.6, 137.9, 196.9.
7δ 2.96-3.00 (2H, m), 3.14-3.18 (2H, m), 3.26 (2H, t, J = 6.7 Hz), 3.50 (2H, t, J = 6.9 Hz), 7.20-7.33 (5H, m), 7.58 (1H, dd, J = 8.4, 2.4 Hz ), 7.73 (1H, d, J = 2.2 Hz), 7.82 (1H, d, J = 8.4 Hz ), 9.20 (1H, br s).δ 32.2, 38.9, 42.1, 48.4, 127.4, 128.4, 129.30, 129.31, 130.9, 132.01, 132.05, 136.5, 137.4, 137.9, 198.4.
8δ 3.12-3.16 (2H, m), 3.37-3.45 (2H, m), 3.57-3.61 (2H, m), 3.80 (2H, t, J = 6.7 Hz), 7.22-7.35 (5H, m), 8.24 (2H, d, J = 8.7 Hz), 8.37 (2H, d, J = 9.1 Hz), 11.09 (1H, br s).δ 30.0, 33.8, 47.8, 54.3, 124.5, 127.4, 129.2, 129.5, 130.1, 137.7, 141.3, 150.8, 196.6.
9δ 3.08-3.58 (8H, m), 6.88 (2H, d, J = 8.7 Hz), 7.21-7.34 (5H, m), 7.89 (2H, d, J = 8.7 Hz), 10.57(1H, s), 10.60 (1H, br s).δ 30.0, 32.5, 48.3, 54.2, 115.9, 127.4, 128.3, 129.2, 129.5, 131.3, 137.7, 163.2, 195.4.
10δ 2.95-2.99 (2H, m), 3.14-3.18 (2H, m), 3.26 (2H, t, J = 6.9 Hz), 3.48 (2H, t, J = 6.9 Hz), 7.22-7.34 (5H, m), 7.98 (2H, dd, J = 4.0, 1.1 Hz), 8.05 (2H, dd, J = 4.7, 1.1 Hz), 9.20 (1H, br s).δ 32.2, 35.4, 42.3, 48.4, 127.4, 129.31, 129.33, 129.6, 134.5, 136.1, 137.9, 143.4, 190.5.
Table 2. UV, IR and MS results for the synthesized compounds.
Table 2. UV, IR and MS results for the synthesized compounds.
CompoundMWUV*MS m/z M+IR (KBr, cm-1)
λmaxlogεC(M)C=O stretching
1289.802444.125.1x10-5253.31678
2303.832554.26.58x10-5267.71677
3319.832863.8112.5x10-5283.41655
4324.242534.213.8x10-5287.71675
5307.792454.036.5x10-5271.61671
6368.702584.285.4x10-5331.01672
7427.582483.8513.9x10-5321.31701
8334.802624.334.48x10-5298.51695
9305.802834.483.2x10-5269.31666
10295.83262, 2873.95, 3.8613.5x10-5259.41651
*Except for compound 8, all UV spectra were recorded in ethanol.

Product characterization

The characterization of compound 1 is presented in some detail as a representative example. In the 1H-NMR spectrum of compound 1 (Table 1), the protons of the two methylene groups located on each side of the nitrogen atom were observed as multiplets (2 x 2 H) at δ 2.96-3.17 and 3.18-3.21 ppm, while the two protons of the methylene located next to the phenyl ring were observed as a triplet at δ 3.28 ppm (J = 6.7 Hz) and the two protons of the methylene located next to the carbonyl group were observed as a triplet at δ 3.55 ppm (J = 6.9 Hz). The phenyl ring aromatic protons were observed as multiplets at δ 7.22-7.34 ppm (5 H), δ 7.53-7.69 ppm (3 H) and δ 7.96-7.98 ppm (2 H). A broad singlet at δ 9.23 ppm (1 H) suggested an NH proton, in accordance with the proposed chemical structure. In the 13C-NMR spectrum of compound 1 (Table 1), methylene carbons at δ 32.2, 35.1, 42.4 and 48.4 ppm, aromatic carbons at δ 127.4, 128.6, 129.31, 129.34, 129.5, 134.4, 136.5 and 137.9 ppm and a carbonyl carbon at δ 197.6 ppm were observed, all in agreement with the proposed structure. In the IR spectrum of compound 1 (Table 2) a strong absorption peak at 1678 cm-1was observed, also suggesting the presence of a carbonyl group. The UV spectrum of compound 1, taken in ethanol (Table 2), gave a λmax peak at 244 nm, also in agreement with the proposed structure. The mass spectrum of compound 1 (Table 2) gave a signal at 253.3, which corresponds to the m/z value of the basic form of compound 1, thus confirming the chemical structure of 1.

Synthetic condition optimization

Using compounds 1 and 10 as representative compounds, different reaction conditions were tested to optimize the synthetic conditions for the whole series. These experiments are summarized in Table 3.
Table 3. Optimization of reaction conditions.
Table 3. Optimization of reaction conditions.
EntryTarget compoundReagent ratioaSolventAcidTime Yield (%)
A11:1.2:1EthanolYes7 hrs95
B101:1.2:1EthanolYes7 hrs93
C11:1.2:1EthanolNo13 hrs95
D101:1.2:1EthanolNo19 hrs96
E12:2:1EthanolYes8 hrs74
F102:2:1EthanolYes14 hrs69
G12:2:1EthanolNo7 hrs0
H102:2:1EthanolNo16 hrs74
I12:2:1NoneNob18c
J102:2:1NoneNob16d
K11:1.2:1NoneNob37
L101:1.2:1NoneNob47
  • a Ketone:paraformaldehyde:phenethylamine hydrochloride
  • b Reaction mixture heated to 83-86°C (ca. 10 minutes); then an exothermic reaction ocurred (see text for details)
  • c 15 % of 1-phenethyl-3-benzoyl-4-phenyl-4-piperidinol was also formed
  • d 21 % of 1-phenethyl-3-(2-thienylcarbonyl)-4-(2-thienyl)-4-piperidinol was also formed
When the syntheses of these two compounds were carried out under the reaction conditions described in the Experimental, i.e. by refluxing a 1:1.2:1 mixture of ketone (acetophenone for 1 and 2-acetylthiophene for 10), paraformaldehyde and phenethylamine hydrochloride in ethanol which was acidified with hydrochloric acid, compounds 1 and 10 were obtained in yields of 95% and 93%, respectively (Table 3, entries A, B). On the other hand, when compounds 1 and 10 were prepared by refluxing the reactants in the same 1:1.2:1 mol ratio in ethanol without acid (entries C, D), compound 1 was still obtained in 95% yield, while compound 10 was obtained in slightly better yield (96%). Although the yields of compounds 1 and 10 were therefore essentially unchanged in the presence or absence of acid, in order to obtain these similar yields under the latter conditions the reflux periods had to be extended by six hours for 1 (almost twice as long) and 12 hours (almost three times as long) for 10. From this it may be concluded that the acidic medium helps to decrease the reaction period by increasing the reaction rate.
When compounds 1 and 10 were prepared in ethanol acidified with concentrated hydrochloric acid but using the appropriate ketone, paraformaldehyde and phenethylamine hydrochloride in a 2:2:1 mol ratio, the reflux periods were eight hours for 1 and 14 hours for 10 and the yields of the reactions were 74 and 69%, respectively (entries E, F), that is, compared with the reactions in the same reaction medium in which the mol ratio of the reactants was 1:1.2:1 (entries A, B), the reaction yields decreased by 21% for compound 1, while reaction period was almost the same, while the yield of compound 10 decreased 24 %, while the reaction period increased two-fold. These results suggested that the best results in terms of reaction yields and especially the reaction time could be achieved by carrying out the reactions in refluxing ethanol acidified with concentrated hydrochloric acid and using of a 1:1.2:1 mol ratio of ketone, paraformaldehyde and phenethylamine hydrochloride.
Compound 1 could not be synthesized when the reactions were run in ethanol without acid using a 2:2:1 mol ratio of reagents, even when the reaction mixture was refluxed for seven hours (entry G). Under these conditions, formation of compound 10 only started after two hours and its production reached its maximum after 16 hours, at which time the reaction was stopped and compound 10 was isolated in 74 % yield (entry H). When these results are compared with those obtained using the same 2:2:1 mol ratio of the reactants but in an acidic ethanolic reaction medium (entries E, F), it is noted that the acidity of the reaction medium seems to be crucial for the synthesis of compound 1, whereas in the case of compound 10, although the reaction time was slightly longer, the yield of the reaction increased by only 5%. When the representative compounds 1 and 10 were synthesized by heating the reactants in 2:2:1 mol ratio in an oil bath in the absence of any solvent and acid (entries I, J), an exothermic reaction was observed after approximately 10 minutes, when the reaction mixture reached a temperature of 83-86 oC, and the products melted giving a homogeneous mixture. Even if the heat source was removed at this point, the exotherms reached 100 °C (entry I) and 108 °C (entry J). When the reaction mixtures had cooled sufficiently ethyl acetate was added and stirring was continued for 17 hrs, to give 1 and 10 in 18 % and 16 % yield, respectively. Under these conditions additional reaction products were also obtained (1-phenethyl-3-benzoyl-4-phenyl-4-piperidinol in addition to compound 1 and 1-phenethyl-3-(2-thienylcarbonyl)-4-(2-thienyl)-4-piperidinol in addition to compound 10, in yields of 15 and 21%, respectively). The production of these piperidinol type byproducts in addition to the desired compounds 1 and 10 was the main difference of this method from the others tried. Comparing the yields of the reactions in which a 2:2:1 mol ratio of reagents was used but without solvent (entries I, J) with the yields using the same ratio of the reactants in acidified ethanol (entries E, F), it is observed that the yield of compound 1 in acidified ethanol (74%) decreased to 18% without solvent, and similarly, the yield of compound 10 decreased from 69% to 16%. When the yields of the reactions without solvent and the reactants in 2:2:1 mol ratio (entries I, J) are compared with the corresponding yields of the reactions with the same ratio of the reactants in ethanol without acid (entries G, H), it was observed that reaction yield in ethanol medium (0%) increased to 18% for compound 1 in a medium without solvent. In case of compound 10, the reaction yield with the ethanol medium (74%) decreased to 16% in a medium without solvent. On the other hand, when the reaction conditions without solvent and a 2:2:1 mol ratio of reactants (entries I, J) are compared with the conditions without solvent but with a 1:1.2:1 mol ratio of reactants (entries K, L), the yields improved from 18% to 37% for 1 and from 16% to 47% for 10 under the latter conditions, as piperidinol byproduct formation is no longer possible with this stoichiometry.
The ratios of ketone, paraformaldehyde amine HCl previously used [16] in the preparation of compound 3 were 1:1.7:1.5, and the paraformaldehyde was added in two portions, which differs from our method. The medium was acidified with HCl after heating for 7 h. First the basic form of compound 3 was obtained using 50% NaOH, and then compound 3 itself was obtained by passing gaseous HCl through a solution of the base in ether. The yield was 64% after purification by crystallization. The reason for a lower yield than that obtained by our method (94%) could be the formation of byproducts such as α,β-unsaturated ketone and piperidinol type compounds, due to deamination of compound 3 in the NaOH solution used [10,13]. In the preparation of compounds 4 and 5 the ratios of ketone, paraformaldehyde and amine HCl previously used were 1:1.7:1 and the reaction mixtures were heated in acidic isopropanol [28]. The compounds were purified by crystallization and obtained in yields of 35% to 40%, respectively. The difference in the reaction solvent (isopropanol vs. ethanol used in our study) might have affected the yield of the reaction.

Conclusions

In conclusion, the syntheses in high reaction yields between 87-98 % and the spectral data of the compounds 1- 10, which are potential bioactive compounds, are reported for the first time in detail. Of these compounds, 2, 6-8, and 10 are new. The optimum reaction conditions for the synthesis of the 1-aryl-3-phenethylamino-1-propanone hydrochloride mono Mannich bases reported in this study were investigated by changing the mol ratios of reactants, solvent and acidity level, using 1 and 10 as representative compounds, It was observed that the most suitable mol ratio of ketone, paraformaldehyde and phenethylamine hydrochloride reactants was 1:1.2:1 (compared with 2:2.1) and the most suitable reaction medium was ethanol containing concentrated hydrochloric acid (compared with the reaction without solvent and using only ethanol). This study may serve as a guide for the synthesis conditions of the compounds with similar chemical structures.

Experimental

General

Chemicals used in this study were as follows: acetophenone, 4’-hydroxyacetophenone (Merck, Hohenbrunn, Germany), 4’-methylacetophenone, 4’-nitroacetophenone, 4’-chloroacetophenone, 2-acetylthiophene (Fluka, Steinheim, Switzerland), 4’-methoxyacetophenone, 4’-fluoroacetophenone, 4’-bromoacetophenone, 2’,4’-dichloroacetophenone (Acros, Geel, Belgium), paraformaldehyde (Merck, Darmstadt, Deutschland), methanol, ethyl acetate (Riedel-deHaën, Seelze, Germany) and ethanol (J.T. Baker, Deventer, Holland). The 1H- and 13C-NMR spectra were recorded at 400 (100) MHz on a Varian spectrometer (Danbury, USA). Infrared spectra were obtained for KBr disks on a Mattson 1000 FT-IR spectrophotometer (Cambrige, England). Elemental analyses were carried out with a Leco CHNS-932 instrument (Michigan, USA). EI-MS spectra were recorded on a Thermo-Finnigan mass analyzer (San Jose, USA). UV spectra of compounds were recorded on a Thermo Electron Heλios (α) (UVA 114903) spectrometer (Cambridge, UK). Melting points were measured with an Electrothermal IA 9100 (Essex, UK).

Synthesis of 1-aryl-3-phenethylamino-1-propanone hydrochlorides 1-10

A previously reported Mannich reaction procedure [8,10] was followed. The appropriate ketone, paraformaldehyde and phenethylamine hydrochloride in 1:1.2:1 mol ratio were placed in a reaction flask containing ethanol (5 mL), then HCl (0.5 mL, 36.5 %, w/v) in ethanol (2 mL) was added to this mixture, which was then refluxed for varying periods of time. The reactions were followed by TLC analysis (Merck Art No. 5715: Silica gel 60 F254, 0.25 mm thickness) with 4:1 CHCl3:methanol as eluent and visualization with a UV lamp. Refluxing was continued until the ketone component of the reaction mixture was consumed. The amounts (mmol) of ketone, paraformaldehyde and phenethylamine hydrochloride used, reflux times (hours), crystallisation solvents used, yields of the reactions (%) and melting points of the products (oC) are summarized in Table 4.
Table 4. The amounts of reagent (mmol), reflux times of the reactions (hours), crystallisation solvents, yield of the reactions (%), and the melting points of the compounds (oC).
Table 4. The amounts of reagent (mmol), reflux times of the reactions (hours), crystallisation solvents, yield of the reactions (%), and the melting points of the compounds (oC).
CompoundKetoneParaformaldehydePhenethylamine hydrochlorideTime (hours)Crystallisation solventYield %Melting Point (°C)
18.39.98.37Ethyl acetate95169-171
27.48.97.48Methanol97163-165
36.67.96.613Ethanol94164-165a
46.47.76.416Ethanol96195-197b
57.28.67.212Ethanol87192-194c
65.06.05.014Methanol.97205-207
75.26.35.226Methanol91167-169
86.07.26.08Methanol98194-195
97.38.87.319Methanol93167-169
107.99.57.97Ethanol93156-157
  • a: Melting point for 3 previously reported [16] as 164-166 °C (from methanol).
  • b: Melting point for 4 previously reported [28] as 210 °C (from isopropanol).
  • c: Melting point for 5 previously reported [28] as 200 °C (from acetone).

Acknowledgments

This research was supported by the Ataturk University Research Fund (Project number: 2006/96).

References

  1. Dimmock, J. R.; Kumar, P. Anticancer and cytotoxic properties of Mannich bases. Curr. Med. Chem. 1997, 4, 1–22. [Google Scholar]
  2. Erciyas, E.; Erkaleli, H. I.; Cosar, G. Antimicrobial evaluation of some styryl ketone derivatives and related thiol adducts. J. Pharm. Sci. 1994, 83, 545–548. [Google Scholar] [CrossRef]
  3. Manavathu, E. K.; Vashishtha, S. C.; Alangaden, G. J.; Dimmock, J. R. In vitro antifungal activity of some Mannich bases of conjugated styryl ketones. Can. J. Microbiol. 1998, 44, 74–79. [Google Scholar] [CrossRef]
  4. Gul, H. I.; Denizci, A. A.; Erciyas, E. Antimicrobial evaluation of some Mannich bases of acetophenones and their representative quaternary derivatives. Arzneimittel-Forsch. 2002, 52, 773–777. [Google Scholar]
  5. Gul, H. I.; Ojanen, T.; Hanninen, O. Antifungal evaluation of some bis Mannich bases derived from acetophenone and their corresponding piperidinols and stability studies. Biol. Pharm. Bull. 2002, 25, 1307–1310. [Google Scholar]
  6. Gul, H. I.; Ojanen, T.; Vepsalainen, J.; Gul, M.; Erciyas, E.; Hanninen, O. Antifungal activity of some mono, bis and quaternary Mannich bases derived from acetophenone. Arzneimittel-Forsch. 2001, 51, 72–75. [Google Scholar]
  7. Gul, H. I.; Sahin, F.; Gul, M.; Ozturk, S.; Yerdelen, K. O. Evaluation of antimicrobial activities of several Mannich bases and their derivatives. Arch. Pharm. (Weinheim) 2005, 338, 335–338. [Google Scholar] [CrossRef]
  8. Gul, H. I.; Vepsalainen, J.; Gul, M.; Erciyas, E.; Hanninen, O. Cytotoxic activities of mono and bis Mannich bases derived from acetophenone against Renca and Jurkat cells. Pharm. Acta Helv. 2000, 74, 393–398. [Google Scholar] [CrossRef]
  9. Gul, M.; Gul, H. I.; Vepsalainen, J.; Erciyas, E.; Hanninen, O. Effect of acetophenone derived Mannich bases on cellular glutathione level in Jurkat cells: a possible mechanism of action. Arzneimittel-Forsch. 2001, 51, 679–682. [Google Scholar]
  10. Gul, H. I.; Gul, M.; Erciyas, E. Syntheses and stability studies of some Mannich bases of acetophenones and evaluation of their cytotoxicity against Jurkat cells. Arzneimittel-Forsch. 2002, 52, 628–635. [Google Scholar]
  11. Gul, H. I.; Gul, M.; Hänninen, O. Cytotoxic activities of some mono and bis Mannich bases derived from acetophenone in brine shrimp bioassay. Arzneimittel-Forsch. 2002, 52, 840–843. [Google Scholar]
  12. Gul, H. I.; Gul, M.; Vepsalainen, J.; Erciyas, E.; Hanninen, O. Cytotoxicity of some azines of acetophenone derived mono-Mannich bases against Jurkat cells. Biol. Pharm. Bull. 2003, 26, 631–637. [Google Scholar]
  13. Gul, M.; Gul, H. I.; Das, U.; Hanninen, O. Biological evaluation and structure-activity relationships of bis-(3-aryl-3-oxo-propyl)-methylamine hydrochlorides and 4-aryl-3-arylcarbonyl-1-methyl-4-piperidinol hydrochlorides as potential cytotoxic agents and their alkylating ability towards cellular glutathione in human leukemic T cells. Arzneimittel-Forsch. 2005, 55, 332–337. [Google Scholar]
  14. Gul, M.; Atalay, M.; Gul, H. I.; Nakao, C.; Lappalainen, J.; Hanninen, O. The effects of some Mannich bases on heat shock proteins HSC70 and GRP75, and thioredoxin and glutaredoxin levels in Jurkat cells. Toxicol. In Vitro 2005, 19, 573–580. [Google Scholar] [CrossRef]
  15. Suleyman, H.; Gul, H. I.; Gul, M.; Alkan, M.; Gocer, F. Anti-inflammatory activity of bis(3-aryl-3-oxo-propyl)methylamine hydrochloride in rat. Biol. Pharm. Bull. 2007, 30, 63–67. [Google Scholar]
  16. Atwal, M. S.; Bauer, l.; Dixit, S. N.; Gearien, J. E.; Megahy, M.; Morris, R.; Pokorny, C. Analgetics. II. Relationship between structure and activity of some beta-aminoketones. J. Med. Chem. 1969, 12, 994–997. [Google Scholar] [CrossRef]
  17. Chen, H.; Ji, Z.; Wong, L. K.; Siuda, J. F.; Narayanan, V. L. Synthesis, antiinflammatory, and cytotoxic activities of 2-alkyl and 2-benzyl-2-dimethylaminomethyl-5-(E)-arylidene cyclo-pentanone hydrochlorides. Pharm. Res. 1996, 13, 1482–1487. [Google Scholar] [CrossRef]
  18. Suleyman, H.; Gul, H. I.; Asoglu, M. Antiinflammatory activity of 3-benzoyl-1-methyl-4-phenyl-4-piperidinol hydrochloride. Pharmacol. Res. 2003, 47, 471–475. [Google Scholar] [CrossRef]
  19. Koechel, D. A.; Rankin, G. O. Diuretic activity of Mannich base derivatives of ethacrynic acid and certain ethacrynic acid analogues. J. Med. Chem. 1978, 21, 764–769. [Google Scholar] [CrossRef]
  20. Lee, C. M.; Plattner, J. J.; Ours, C. W.; Horrom, B. W.; Smital, J. R.; Pernet, A. G.; Bunnell, P. R.; El-Masry, S. E.; Dodge, P. W. [[(Aminomethyl)aryl]oxy]acetic acid esters. A new class of high-ceiling diuretics. 1. Effects of nitrogen and aromatic nuclear substitution. J. Med. Chem. 1984, 27, 1579–1587. [Google Scholar] [CrossRef]
  21. Borenstein, M. R.; Doukas, P. H. Anticonvulsant activity of indanylspirosuccinimide Mannich bases. J. Pharm. Sci. 1987, 76, 300–302. [Google Scholar] [CrossRef]
  22. Dimmock, J. R.; Jonnalagadda, S. S.; Phillips, O. A.; Erciyas, E.; Shyam, K.; Semple, H. A. Anticonvulsant properties of some Mannich bases of conjugated arylidene ketones. J. Pharm. Sci. 1992, 81, 436–440. [Google Scholar] [CrossRef]
  23. Gursoy, A.; Karali, N.; Buyuktimkin, S.; Demirayak, S.; Ekinci, A. C.; Ozer, H. Some 3-hydrazono-2-indolinones and N-Mannich bases as potential anticonvulsants. Farmaco 1996, 51, 437–442. [Google Scholar]
  24. Gul, H. I.; Calis, U.; Vepsalainen, J. Synthesis and evaluation of anticonvulsant activities of some bis-Mannich bases and corresponding piperidinols. Arzneimittel-Forsch. 2002, 52, 863–869. [Google Scholar]
  25. Gul, H. I.; Calis, U.; Vepsalainen, J. Synthesis of some mono-Mannich bases and corresponding azine derivatives and evaluation of their anticonvulsant activity. Arzneimittel-Forsch. 2004, 54, 359–364. [Google Scholar]
  26. Gul, M.; Gul, H. I.; Hanninen, O. Effects of Mannich bases on cellular glutathione and related enzymes of Jurkat cells in culture conditions. Toxicol. In Vitro 2002, 16, 107–112. [Google Scholar] [CrossRef]
  27. Roth, H. J.; Schwarz, D.; Hundeshagen, G. Photoreactions of phthalimide Mannich bases in methanol. Arch. Pharm. (Weinheim) 1976, 309, 48–52. [Google Scholar] [CrossRef]
  28. Collino, F.; de-Nardo, M. Mannich ketobases with narcotic antagonist activity. Boll. Chim. Farm. 1983, 122, 393–404. [Google Scholar]
  29. Prost, M.; Urbain, M.; Charlier, R. 3-Amino-4'-Hydroxybutyrophenones. Belg. Chim. Therapeut. 1970, 5, 303–311. [Google Scholar]
  • Sample Availability: Samples of compounds 1-10 are available from the authors.

Share and Cite

MDPI and ACS Style

Mete, E.; Gul, H.I.; Kazaz, C. Synthesis of 1-Aryl-3-phenethylamino-1-propanone Hydrochlorides as Possible Potent Cytotoxic Agents. Molecules 2007, 12, 2579-2588. https://doi.org/10.3390/12122579

AMA Style

Mete E, Gul HI, Kazaz C. Synthesis of 1-Aryl-3-phenethylamino-1-propanone Hydrochlorides as Possible Potent Cytotoxic Agents. Molecules. 2007; 12(12):2579-2588. https://doi.org/10.3390/12122579

Chicago/Turabian Style

Mete, Ebru, Halise Inci Gul, and Cavit Kazaz. 2007. "Synthesis of 1-Aryl-3-phenethylamino-1-propanone Hydrochlorides as Possible Potent Cytotoxic Agents" Molecules 12, no. 12: 2579-2588. https://doi.org/10.3390/12122579

Article Metrics

Back to TopTop