Supramolecular Complex Formation by β-Cyclodextrin and Ferrocenylnaphthalene Diimide-intercalated Double Stranded DNA and Improved Electrochemical Gene Detection

doi:10.3390/10060693

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Molecules 2005, 10(6), 693-707; doi:10.3390/10060693

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Supramolecular Complex Formation by β-Cyclodextrin and Ferrocenylnaphthalene Diimide-intercalated Double Stranded DNA and Improved Electrochemical Gene Detection

Shinobu Sato, Takahiko Nojima, Michinori Waki and Shigeori Takenaka*

Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan

* Author to whom correspondence should be addressed.

Received: 16 November 2004 / Accepted: 29 December 2004 / Published: 14 July 2005

(This article belongs to the Special Issue Synthesis and Chemistry of Chiral Metallocenes)

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Abstract: Ferrocenylnaphthalene diimide 1 can bind to double stranded DNA (dsDNA) by the threading intercalation mode and the resulting complex was stabilized further by β- cyclodextrin (CD) by forming a supramolecular complex. These complex formation processes were studied by spectroscopic, viscometric, and electrochemical means in the absence or presence of β-CD. Quantitative analysis by quartz crystal microbalance (QCM) and electrochemical experiments strongly suggested a 2:1 binding stoichiometry for β-CD to 1 threading-intercalated to the dsDNA-immobilized electrode. Owing to this supramolecular complex formation, electrochemical DNA detection based on 1 was improved considerably.

Keywords: Ferrocenylnaphthalene diimide; β-cyclodextrin (CD); DNA; electrochemical detection; supramolecular complex

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MDPI and ACS Style

Sato, S.; Nojima, T.; Waki, M.; Takenaka, S. Supramolecular Complex Formation by β-Cyclodextrin and Ferrocenylnaphthalene Diimide-intercalated Double Stranded DNA and Improved Electrochemical Gene Detection. Molecules 2005, 10, 693-707.

AMA Style

Sato S, Nojima T, Waki M, Takenaka S. Supramolecular Complex Formation by β-Cyclodextrin and Ferrocenylnaphthalene Diimide-intercalated Double Stranded DNA and Improved Electrochemical Gene Detection. Molecules. 2005; 10(6):693-707.

Chicago/Turabian Style

Sato, Shinobu; Nojima, Takahiko; Waki, Michinori; Takenaka, Shigeori. 2005. "Supramolecular Complex Formation by β-Cyclodextrin and Ferrocenylnaphthalene Diimide-intercalated Double Stranded DNA and Improved Electrochemical Gene Detection." Molecules 10, no. 6: 693-707.

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