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Medicina 2018, 54(4), 54; https://doi.org/10.3390/medicina54040054

Serum and Tissue HIF-2 Alpha Expression in CIN, N-Acetyl Cysteine, and Sildenafil-Treated Rat Models: An Experimental Study

1
Department of Emergency Medicine, Kayseri Training and Research Hospital, 38010 Kayseri, Turkey
2
Department of Biochemistry, Kayseri Training and Research Hospital, 38010 Kayseri, Turkey
3
Experimantal Research Center, Erciyes University, 38010 Kayseri, Turkey
4
Department of Histology and Embryology, University of Erciyes, 38010 Kayseri, Turkey
5
Department of Biostatistics, Erciyes University Faculty of Medicine, 38010 Kayseri, Turkey
*
Author to whom correspondence should be addressed.
Received: 25 June 2018 / Revised: 26 July 2018 / Accepted: 26 July 2018 / Published: 30 July 2018
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Abstract

Background and Objectives: Contrast-induced nephropathy (CIN), is acute renal damage due to contrast agents. This study is conducted to evaluate serum and renal heterodimeric nuclear transcription factor (HIF)-2 alpha levels and its tissue expression in contrast-induced nephropathy, and in N-acetyl cysteine (NAC)-and Sildenafil-treated rat models. Materials/Methods: This randomized, controlled, interventional animal study was conducted on Wistar rats. Rats (n = 36) were randomly assigned to four groups: control (n = 9), CIN group (n = 9), CIN + NAC group (n = 9), and sildenafil (n = 9). The rat model was used to form iohexol-originated CIN. During the modeling, prophylactic treatment was performed at the 24th and 48th h. After 48 h of modeling, blood, urine, and tissue samples were obtained for biochemical analyses. HIF-2-α levels were measured in renal tissue, serum, and urine samples. Renal sections were also performed for histopathologic and immunohistochemical evaluations of renal injury and HIF-2-α expression. Results: In the CIN model, HIF-2α levels and other biochemical parameters were significantly increased (p < 0.01). Both sildenafil and NAC efficiently decreased renal damage due to contrast agents, as shown in histopathologic examinations (p < 0.05). Similarly, after treatment with sildenafil and NAC, HIF-2α levels were significantly decreased (p < 0.05). Conclusions: The current study shows that serum and tissue HIF-2α levels decrease in CIN. Besides, the levels and tissue expression of HIF-2α decrease with both NAC and sildenafil treatments. With further studies, HIF-2α can be investigated as a biomarker of CIN and can be used in the follow-up of patients with CIN. View Full-Text
Keywords: contrast-induced nephropathy; hypoxia-inducible factor 2α; N-acetyl-cysteine; sildenafil contrast-induced nephropathy; hypoxia-inducible factor 2α; N-acetyl-cysteine; sildenafil
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Altintop, I.; Tatli, M.; Karakukcu, C.; Soyer Sarica, Z.; Hanım Yay, A.; Balcioglu, E.; Ozturk, A. Serum and Tissue HIF-2 Alpha Expression in CIN, N-Acetyl Cysteine, and Sildenafil-Treated Rat Models: An Experimental Study. Medicina 2018, 54, 54.

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