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Medicina 2018, 54(1), 1; https://doi.org/10.3390/medicina54010001

Effects of Silymarin-Loaded Nanoparticles on HT-29 Human Colon Cancer Cells

1
Cell & Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61335, Iran
2
Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61335, Iran
3
Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61335, Iran
4
Department of Pharmaceutics, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61335, Iran
*
Author to whom correspondence should be addressed.
Received: 6 July 2017 / Revised: 26 February 2018 / Accepted: 26 February 2018 / Published: 9 March 2018
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Abstract

Background and objective: Previous studies have demonstrated the anti-cancer effects of silymarin (SLM). However, the low bioavailability of SLM has restricted its use. This study investigated the toxic effect of nanostructured SLM encapsulated in micelles (Nano-SLM) on the growth of the HT-29 human colon cancer cell line. Materials and methods: HT-29 cells were treated with 25 μM/mL of SLM or Nano-SLM for 48 h. MTT and colony formation assays were used to assess the cytotoxicity and proliferation of HT-29 cells, respectively. The cells were stained with annexin V/PI for assessment of apoptosis. Results: MTT assays revealed that Nano-SLM treatment was able to exert a more pronounced toxic effect on the HT-29 cells as compared to free SLM treatment (p < 0.01). In the Nano-SLM-treated cells, colony numbers were significantly reduced in comparison to the free SLM-treated cells (p < 0.01). Apoptotic and necrotic indexes of Nano-SLM-treated HT-29 cells were also significantly increased in comparison to those of the free SLM-treated cells (p < 0.01). The viability, proliferation and apoptosis of healthy cells (NIH-3T3 cells) were not changed in response to Nano-SLM or SLM. Conclusions: Our results indicate that Nano-SLM enhances the anti-cancer effects of SLM against human colon cancer cells. View Full-Text
Keywords: silymarin; micelle; colon cancer; nanodrug silymarin; micelle; colon cancer; nanodrug
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Mombeini, M.; Saki, G.; Khorsandi, L.; Bavarsad, N. Effects of Silymarin-Loaded Nanoparticles on HT-29 Human Colon Cancer Cells. Medicina 2018, 54, 1.

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