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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
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Medicina 2017, 53(3), 203-210; https://doi.org/10.1016/j.medici.2017.07.001 (registering DOI)

Scaffold design for artificial tissue with bone marrow stem cells

1
Institute of Physiology and Pharmacology, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania
2
Institute of Cardiology, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania
3
Laboratory of Immunology, National Cancer Institute, Vilnius, Lithuania
4
Institute of Materials Science, Kaunas University of Technology, Kaunas, Lithuania
5
Faculty of Mechanical Engineering and Design, Kaunas University of Technology, Kaunas, Lithuania
*
Author to whom correspondence should be addressed.
Received: 23 January 2017 / Revised: 26 June 2017 / Accepted: 3 July 2017 / Published: 13 July 2017
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Abstract

Objective: The aim of this study was to test polymeric materials (collagen, fibrin, polyimide film, and polylactic acid) for single- and multi-layer scaffold formation.
Materials and methods: In our study, we used rabbit bone marrow stem cells (rBMSCs) and human mesenchymal stem cells (hMSCs) with materials of a different origin for the formation of an artificial scaffold, such as a collagen scaffold, fibrin scaffold produced from clotted rabbit plasma, electrospun poly(lactic acid) (PLA) mats, polyimide film (PI), and the combination of the latter two. Cell imaging was performed 3–14 days after cell cultivation in the scaffolds. Time-lapse imaging was used to determine hMSC mobility on the PI film.
Results: Cell incorporation in collagen and clotted fibrin scaffolds was evaluated after 2-week cultivation in vitro. Histological analysis showed that cells penetrated only external layers of the collagen scaffold, while the fibrin clot was populated with rBMSCs through the entire scaffold thickness. As well, cell behavior on the laser micro-structured PI film was analyzed.
The mobility of hMSCs on the smooth PI film and the micro-machined surface was 20 ± 2 μmm/h and 18 ± 4 μmm/h, respectively. After 3-day cultivation, hMSCs were capable of spreading through the whole 100 ± 10 μmm-thick layer of the electrospun PLA scaffold and demonstrated that the multilayer scaffold composed of PI and PLA materials ensured a suitable environment for cell growth.
Conclusions: The obtained results suggest that electrospinning technology and femtosecond laser micro-structuring could be employed for the development of multi-layer scaffolds. Different biopolymers, such as PLA, fibrin, and collagen, could be used as appropriate environments for cell inhabitation and as an inner layer of the multi-layer scaffold. PI could be suitable as a barrier blocking cell migration from the scaffold. However, additional studies are needed to determine optimal parameters of inner and outer scaffold layers. View Full-Text
Keywords: Biological pacemaker; Scaffold; Stem cells; Bone marrow; Polyimide film Biological pacemaker; Scaffold; Stem cells; Bone marrow; Polyimide film
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Noreikaitė, A.; Antanavičiūtė, I.; Mikalayeva, V.; Darinskas, A.; Tamulevičius, T.; Adomavičiūtė, E.; Šimatonis, L.; Akramienė, D.; Stankevičius, E. Scaffold design for artificial tissue with bone marrow stem cells. Medicina 2017, 53, 203-210.

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